Molecular Biology

, Volume 47, Issue 5, pp 685–691

CD271 enrichment does not help isolating mesenchymal stromal cells from G-CSF-mobilized peripheral blood

  • Iman Ahrari
  • Armin Attar
  • Nima Pourhabibi Zarandi
  • Maryam Zakerinia
  • Mohsen Khosravi Maharlooei
  • Ahmad Monabati
Cell Molecular Biology

DOI: 10.1134/S0026893313050051

Cite this article as:
Ahrari, I., Attar, A., Zarandi, N.P. et al. Mol Biol (2013) 47: 685. doi:10.1134/S0026893313050051
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Abstract

Reports on the isolation of mesenchymal stromal cells (MSCs) from granulocyte colony stimulating factor mobilized peripheral blood (G-CSF-mobilized PB) using regular culturing techniques are controversial. Enrichment techniques such as CD133 isolation have increased the success rates. CD271 is a wellknown marker for enrichment of MSCs from bone marrow (BM). In the present study, we aimed to find out whether CD271 enrichment can help isolation of MSCs from G-CSF-mobiiized PB. Five G-CSF-mobilized PB samples were collected from the remnant parts of the bags used for BM transplantation. Five BM samples were used as the control. Mononuclear cells (MNCs) from both resources were collected and underwent magnetic sorting for CD271-positive cells. The isolated cells were cultured, undergoing flowcytometry and differentiation assays to determine if they fulfill MSCs characteristics. CD271-positive portion of G-CSF-mobilized PB did not yield any cell outgrowth but the BM counterpart could successfully form MSC colonies. Although the percentage of CD271+ cells showed no difference between BM-MNCs and G-CSF-mobilized PB-MNCs, hematopoietic markers such as CD45, CD34 and CD133 composed a higher percentage of CD271-positive cells in the G-CSF-tnobiiized PB group. Results obtained indicated that CD271 enrichment does not help isolation of MSCs from G-CSF-mobilized PB. In this source, almost all of the CD271+ cells are from hematopoietic origin and the frequency of MSCs is so low that possibly during the process of cell isolation most of them are lost and the isolation fails.

Keyword

CD271mesenchymal stromal cellmesenchymal stem cellgranulocyte colony-stimulating factormobilized peripheral blood

Abbreviations

BM

bone marrow

G-CSF-mobilized PB

granulocyte colony-stimulating factor mobilized peripheral blood

MNC

mononuclear cell

MSC

mesenchymal stromal cell

Copyright information

© Pleiades Publishing, Inc. 2013

Authors and Affiliations

  • Iman Ahrari
    • 1
    • 2
  • Armin Attar
    • 1
    • 2
    • 3
  • Nima Pourhabibi Zarandi
    • 1
    • 2
  • Maryam Zakerinia
    • 4
  • Mohsen Khosravi Maharlooei
    • 1
    • 2
  • Ahmad Monabati
    • 5
  1. 1.Student Research CommitteeShiraz University of Medical SciencesShirazIran
  2. 2.Cell and Molecular Medicine ClubShiraz University of Medical SciencesShirazIran
  3. 3.Department of Cardiovascular MedicineShiraz University of Medical SciencesShirazIran
  4. 4.Department of Internal Medicine, Hematology and Oncology DivisionShiraz University of Medical SciencesShirazIran
  5. 5.Department of PathologyShiraz University of Medical SciencesShirazIran