Abstract
The applicability of real-time PCR amplification of the chromosome Y marker DYS14 for sex determination was studied. With this aim, real-time PCR of DYS14 (located within the TSPY1-encoding gene) was performed in plasma DNA specimens obtained from 30 men and 30 women. The PCR results showed that 30 specimens were of male and the other 30 were of female origin. All the results were confirmed by the tests for the SRY marker conventionally used in forensic examination. The detection limit for the DYS14-containing DNA region was established in dilution experiments and was equal to 6.7 pg of DNA (two copies of the genome), which corresponds to 6.7 ng of DNA (2000 copies of the genome) in 1 ml of blood. This level of sensitivity allows sex determination in specimens with small amounts of genetic material. The method can be used for noninvasive prenatal diagnostics of sex-linked congenital diseases and in forensic medical examination.
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References
Dadali E.L., Baryshnikova N.V. 2006. Diseases with Unconventional Types of Inheritance. In: Genetika (Genetics). Moscow: Akademkniga, pp. 468–500.
Hsu D.T. 2010. Cardiac manifestations of neuromuscular disorders in children. Paediatr. Respir. Rev. 11, 35–38.
Dati E., Baldinotti F., Conidi M.E., et al. 2009. A girl with Tomboy behavior: Lesson from misdiagnosis in a baby with ambiguous genitalia. Sex Dev. (in press).
Barkov I.Yu., Bakharev V.A., Karetnikova N.A. 1999. Prenatal sex determination (a review). Probl. Reprod. 1, 5–14.
Evans M.I., Wapner R.J. 2005. Invasive prenatal diagnostic procedures. Semin. Perinatol. 29, 215–218.
Ajayi G.O. 2009. Chorionic villus sampling: Analysis of the first 350 singleton pregnancies by a single operator. Clin. Exp. Obstet. Gynecol. 36, 251–253.
Kong C.W., Leung T.N., Leung T.Y., et al. 2006. Risk factors for procedure-related fetal losses after mid-trimester genetic amniocentesis. Prenatal Diagn. 26, 925–930.
Willruth A., Vieten J., Berg C., et al. 2010. Decision making and attitudes towards invasive prenatal diagnosis in the early second trimester. Ultraschall. Med. (in press).
Nakata N., Wang Y., Bhatt S. 2010. Trends in prenatal screening and diagnostic testing among women referred for advanced maternal age. Prenatal Diagn. 30, 198–206.
Sekido R. 2010. SRY: A transcriptional activator of mammalian testis determination. Int. J. Biochem. Cell Biol. 42, 417–420.
Zimmermann B.G., Maddocks D.G., Avent N.D. 2008. Quantification of circulatory fetal DNA in the plasma of pregnant women. In: Prenatal Diagnosis). Totowa, NJ: Humana Press, pp. 219–231.
Floriano-Sánchez E., Cárdenas-Rodríguez N., Castro-Marín M., et al. 2009. DD3(PCA3) gene expression in cancer and prostatic hyperplasia. Clin. Invest. Med. 32, E258.
Tamkovich S.N., Vlasov V.V., Laktionov P.P. 2008. Circulating deoxyribonucleic acids in blood and their using in medical diagnostics. Mol. Biol. 42, 12–23.
Farina A., LeShane E.S., Lambert-Messerlian G.M., et al. 2003. Evaluation of cell-free fetal DNA as a second-trimester maternal serum marker of Down syndrome pregnancy. Clin. Chem. 49, 239–242.
Zhong X.Y., Holzgreve W., Tercanli S., et al. 2006. Cell-free foetal DNA in maternal plasma does not appear to be derived from the rich pool of cell-free foetal DNA in amniotic fluid. Arch. Gynecol. Obstet. 273, 221–226.
Lo Y.M.D. 2008. Fetal nucleic acids in maternal plasma. Ann. N.Y. Acad. Sci. 1137, 140–143.
Lo Dennis Y.M., Chiu R.W.K. 2007. Prenatal diagnosis: progress through plasma nucleic acids. Nature Rev. Genet. 8, 71–77.
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Original Russian Text © E.G. Blagodatskikh, A.G. Nikitin, Yu.A. Seregin, K.A. Blagodatskikh, V.V. Nosikov, 2010, published in Molekulyarnaya Biologiya, 2010, Vol. 44, No. 4, pp. 646–649.
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Blagodatskikh, E.G., Nikitin, A.G., Seregin, Y.A. et al. Sex determination in biological specimens using the DYS14 marker. Mol Biol 44, 568–570 (2010). https://doi.org/10.1134/S0026893310040102
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DOI: https://doi.org/10.1134/S0026893310040102