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Mutations in the DNA polymerase and thymidine kinase genes of herpes simplex virus clinical isolates resistant to antiherpetic drugs

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Abstract

Primary structures of DNA polymerase (ul30) and thymidine kinase (ul23) genes from several herpes simplex virus type 1 (HSV-1) clinical isolates di ffering in sensitivity to several antiherpetic drugs were determined and compared to those of two laboratory HSV-1 strains one of which (L2) was sensitive and the other (L2/R) was resistant to acyclovir. The phylogenetic sequence analysis showed that the ul30 and ul23 sequences of clinical isolates were close to those of L2, and that ul30 conserved regions differed between HSV-1 isolates and L2 only in point mutations and degenerated substitutions. Several new mutations in the HSV-1 DNA polymerase and thymidine kinase functional domains were identified as substitutions associated with strain resistance to ACV and other antiherpetic drugs.

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Authors and Affiliations

  1. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991, Russia

    A. N. Korovina, S. N. Kochetkov & M. K. Kukhanova

  2. Research Center for Medical Genetics, Russian Academy of Medical Sciences, Moscow, 115478, Russia

    A. A. Gus’kova & M. Yu. Skoblov

  3. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, Moscow, 123098, Russia

    V. L. Andronova & G. A. Galegov

  4. Shemyakin & Ovchinnikov Institute ofBioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia

    Yu. S. Skoblov

Authors
  1. A. N. Korovina
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  2. A. A. Gus’kova
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  3. M. Yu. Skoblov
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  4. V. L. Andronova
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  5. G. A. Galegov
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  6. S. N. Kochetkov
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  7. M. K. Kukhanova
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  8. Yu. S. Skoblov
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Correspondence to A. N. Korovina.

Additional information

Original Russian Text © A.N. Korovina, A.A. Gus’kova, M.Yu. Skoblov, V.L. Andronova, G.A. Galegov, S.N. Kochetkov, M.K. Kukhanova, Yu.S. Skoblov, 2010, published in Molekulyarnaya Biologiya, 2010, Vol. 44, No. 3, pp. 488–496.

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Korovina, A.N., Gus’kova, A.A., Skoblov, M.Y. et al. Mutations in the DNA polymerase and thymidine kinase genes of herpes simplex virus clinical isolates resistant to antiherpetic drugs. Mol Biol 44, 431–438 (2010). https://doi.org/10.1134/S0026893310030118

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  • DOI: https://doi.org/10.1134/S0026893310030118

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