Molecular Biology

, Volume 41, Issue 4, pp 665–669

Development of a biochip with an internal calibration curve for quantitating two forms of the prostate-specific antigen

  • E. V. Konovalova
  • E. N. Savvateeva
  • E. I. Dementieva
  • M. A. Filippova
  • A. Yu. Turygin
  • T. V. Osipova
  • T. P. Ryabykh
  • A. Yu. Rubina
  • A. S. Zasedatelev
Applied Molecular Biology

DOI: 10.1134/S0026893307040218

Cite this article as:
Konovalova, E.V., Savvateeva, E.N., Dementieva, E.I. et al. Mol Biol (2007) 41: 665. doi:10.1134/S0026893307040218

Abstract

Three-dimensional gel-based biological microchips were developed for simultaneous quantitation of total (PSAtot) and free (PSAfree) forms of the prostate-specific antigen in human serum in the “one patient, one biochip” format. A method not demanding construction of calibration curves prior to the assay was applied to quantitation of PSAtot and PSAfree. In addition to gel elements with immobilized antibodies against PSAtot and PSAfree, the biochip contains elements with immobilized PSA at different concentrations, forming an internal calibration curve. Data are processed and interpreted with the special-purpose ImaGelAssay program. The sensitivity of the assay is 0.3 ng/ml for PSAtot and 0.2 ng/ml for PSAfree. The variation coefficient for measurements with one biochip series does not exceed 10%. The correlation coefficients between the estimates obtained for human sera by the biochip assay and by conventional ELISA were 0.988 for PSAtot and 0.987 for PSAfree.

Key words

biochip prostate-specific antigen internal calibration curve simultaneous determination of two PSA forms 

Copyright information

© Pleiades Publishing, Inc. 2007

Authors and Affiliations

  • E. V. Konovalova
    • 1
  • E. N. Savvateeva
    • 1
  • E. I. Dementieva
    • 1
  • M. A. Filippova
    • 1
  • A. Yu. Turygin
    • 1
  • T. V. Osipova
    • 2
  • T. P. Ryabykh
    • 2
  • A. Yu. Rubina
    • 1
  • A. S. Zasedatelev
    • 1
  1. 1.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia
  2. 2.Blokhin Cancer Research CenterRussian Academy of Medical SciencesMoscowRussia