Fractionation of stable carbon isotopes by photoautotrophically growing anoxygenic purple and green sulfur bacteria
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Fractionation of stable carbon isotopes 12C and 13C by three pure cultures of photoautotrophic purple sulfur bacteria (Ectothiorhodospira shaposhnikovii, Lamprocystis purpureus, and Thiocapsa sp.) (PSB) and the green sulfur bacterium Prosthecochloris sp. (GSB) was investigated in 13–15-day experiments. The cultivation was carried out in a luminostat (2000 lx) on mineral media with 1–1.5 g/l NaHCO3 (inoculum) with the subsequent transfer to the medium with up to 10 g/l NaHCO3. For PSB, the difference in the quantitative characteristics of the isotopic composition of suspended carbon (including bacterial cells) and mineral carbon of the medium (Δ13C = δ13Csubstrate − δ13Cbiomass) changed from 15.0 to 34.3‰. For GSB, the range of Δ13C changes was significantly less (18.3–22.7‰). These data suggested the possibility of a pool of soluble mineral carbon in PSB cells. The pool of intracellular mineral carbon was calculated; depending on the PSB species and growth stage, it varied from 0 to 68% of the total cell carbon. The α coefficients reflecting the carbon isotope fractionation by PSB and GBS and calculated from the changes of the bicarbonate carbon isotopic composition in the medium depending on its consumption were 1.029 ± 0.003 and 1.019 ± 0.001, respectively. These α values did not depend on the growth rate. CO2 fixation on ribulose-bisphosphate was shown to be the major factor determining the carbon isotope fractionation by PSB; at the stage of CO2 penetration into the cell, fractionation was insignificant. In GSB, fractionation occurred mostly at CO2 penetration into the cell, while it was insignificant at the stage of carbon dioxide fixation in the reverse TCA cycle. Analysis of the isotopic data of the photosynthesis by PSB and GSB in meromictic lakes also revealed that in PSB-dominated natural communities suspended organic matter was more enriched with light 13C (Δ13C = 23.4−24.6‰) than in the communities with more active GSB (Δ13C = 10.2−14.0‰)
- Gorlenko, V.M., Dubinina, G.A., and Kuznetsov, S.I., Ekologiya vodnykh mikroorganizmov (Ecology of Aquatic Microorganisms), Moscow: Nauka, 1977.
- Gorlenko, V.M., History of the Study of Biodiversity of Photosynthetic Bacteria, Mikrobiologiya, 2004, vol. 73, no. 5, pp. 633–643 [Microbiology (Engl. Transl.), vol. 73, no. 5, pp. 541–550].
- Biebl, H. and Pfenning, N., Anaerobic CO2 Uptake by Phototrophic Bacteria, Arch. Hydrobiol. Beih. Ergeb. Limnol., 1979, vol. 12, pp. 48–58.
- Overmann, J., Beatly, J.T., and Hall, K.J., Photosynthetic Activity and Population Dynamic of Amoebobacter purpureus in a Meromictic Saline Lake, FEMS Microbial. Ecol., 1994, vol. 15, pp. 309–320. CrossRef
- Ivanov, M.V., Rusanov, I.I., Pimenov, N.V., Bairamov, I.T., Yusupov, S.K., Savvichev, A.S., Lein, A.Yu., and Sapozhnikov, V.V., Microbial Processes of the Carbon and Sulfur Cycles in Lake Mogil’noe, Mikrobiologiya, 2001, vol. 70, no. 5, pp. 675–686 [Microbiology (Engl. Transl.), vol. 70, no. 5, pp. 583–593].
- Bondar’, V.A., Gogotova, G.I., and Zyakun, A.M., Carbon Isotope Fractionation by Photoautotrophic Microorganisms with Different Pathways of CO2 Assimilation, Dokl. Akad. Nauk SSSR, 1976, vol. 228, no. 3, pp. 720–722.
- Quandt, L., Gottschalk, G., Ziegler, H., and Stichler, W., Isotope Discrimination by Photosynthetic Bacteria, FEMS Microbiol. Letts., 1977, vol. 1, pp. 125–128. CrossRef
- Sirevag, R., Buchanen, B.B., Berry, J.A., and Troughton, J.H., Mechanisms of CO2 Fixation in Bacterial Photosynthesis Study by the Carbon Isotope Fractionation Technique, Arch. Microbiol., 1977, vol. 112, no. 1, pp. 35–38. CrossRef
- Wong, W.W. and Sackett, W.M., Isotope Fractionation in Photosynthetic Bacteria during Carbon Dioxide Assimilation, Plant Physiol., 1975, vol. 55, no. 3, pp. 475–479. CrossRef
- Zyakun, A.M., Carbon Isotope Fractionation by Microorganisms and Its Application in Biotechnological Research, Doctoral (Biol.) Dissertation, Pushchino, 1994.
- Kondratieva, E.N, Interrelation between Modes of Carbon Assimilation and Energy Production in Phototrophic Purple and Green Bacteria, in Int. Rev. of Biochem. Microbial. Biochem., Quale, J.R., Ed., vol. 21, pp. 117–175.
- Cooper, T.G., Filmer, D., Wishnick, M., and Lane, M.D., The Active Species of “CO2” Utilized by Ribulose Diphosphate Carboxylise, J. Biol. Chem., 1969, vol. 244, pp. 1081–1083.
- Cooper, T.G. and Wood, H.G., The Carboxylation of Phosphoenolpyruvate and Pyruvate. II. The Active Species of “CO2” Utilized by Phosphoenolpyruvate Carboxylize and Pyruvate Carboxylize, J. Biol. Chem., 1971, vol. 246, pp. 5488–5490.
- Mook, W.C., Bemmerson, J.C., and Staverman, W.H., Carbon Isotope Fractionation between Bicarbonate and Gaseous Carbon Dioxide, Earth and Plant Sci. Lett., 1974, vol. 22, pp. 169–176. CrossRef
- Zyakun, A.M, Potential of 13C/12C variations in Bacterial Methane is Assessing Origin of Environmental Methane, in Hydrocarbon Migration and Its Near-Surface Expression, Schumacher, D. and Abrams, M.A., Eds., 1996, AAPG Memoir 66, ch. 25, pp. 341–352.
- Harrison, A.G. and Thode, H.G., Mechanism of the Bacterial Reduction of Sulfate from Isotope Fractionation Studies, Trans. Faraday Society, 1958, vol. 54, pp. 84–92. CrossRef
- Hinga, K.R., Arthur, M.A., Pilson, M.E.Q., and Whitaker, D., Carbon Isotope Fractionation by Marine Phytoplankton in Culture: The Effects of CO2 Concentration, pH, Temperature, and Species, Global Boigeochem. Cycles, 1994, vol. 8, no. 1, pp. 91–102. CrossRef
- Tielmann, J., Tolbert, N.T., Goyal, A., and Senger, H., Two Systems for Concentration CO2 and Bicarbonate during Photosynthesis by Scenedesmus, Plant Physiol., 1990, vol. 92, pp. 622–629. CrossRef
- Fuller, R.C., Smillie, R.M., Sisler, E.C., and Kornberg, H.L., Carbon Metabolism in Chromatium, J. Biol. Chem., 1961, vol. 236, pp. 2140–2149.
- Ivanovskii, R.N., Metabolism of Phototrophic Bacteria under Different Growth Conditions, Doctoral (Biol.) Dissertation, Moscow: Mosk. Gos. Univ., 1986.
- Sirevag, R. and Ormerod, J.G., Carbon Dioxide Fixation in Photosynthetic Green Sulphur Bacteria, Science, 1970, vol. 169, pp. 186–188. CrossRef
- Craig, H., Isotopic Standards for Carbon and Oxygen and Correction Factors for Mass Spectrometric Analysis of Carbon Dioxide, Geochim. Cosmochim. Acta, 1957, vol. 12, pp. 133–149. CrossRef
- Faure, G., Principles of Isotope Geology. 2nd ed., New York: Wiley, 1986.
- Park, R. and Epstein, S., Carbon Isotope Fractionation during Photosynthesis, Geochim. Cosmochim. Acta, 1960, vol. 21, pp. 110–126. CrossRef
- Learu, M.H., Carbon Isotope Fractionation in Plant, Rhutoshemistry, 1981, vol. 20, no. 4, pp. 553–567.
- Fractionation of stable carbon isotopes by photoautotrophically growing anoxygenic purple and green sulfur bacteria
Volume 78, Issue 6 , pp 757-768
- Cover Date
- Print ISSN
- Online ISSN
- SP MAIK Nauka/Interperiodica
- Additional Links
- 12C and 13C fractionation
- photoautotrophic growth
- Ectothiorhodospira shaposhnikovii
- Lamprocystis purpureus
- Thiocapsa sp.
- Prosthecochloris sp.
- indices of fractionation
- δ13C (‰) and ɛ (‰)
- meromictic water bodies
- Industry Sectors
- Author Affiliations
- 1. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, pr. Nauki 5, Pushchino, Moscow oblast, 142290, Russia
- 2. Winogradsky Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia