Experimental Articles

Microbiology

, Volume 78, Issue 4, pp 445-454

First online:

Alkaliphilus peptidofermentans sp. nov., a new alkaliphilic bacterial soda lake isolate capable of peptide fermentation and Fe(III) reduction

  • T. N. ZhilinaAffiliated withWinogradsky Institute of Microbiology, Russian Academy of Sciences Email author 
  • , D. G. ZavarzinaAffiliated withWinogradsky Institute of Microbiology, Russian Academy of Sciences
  • , T. V. KolganovaAffiliated withBioengineering Center, Russian Academy of Sciences
  • , A. M. LysenkoAffiliated withWinogradsky Institute of Microbiology, Russian Academy of Sciences
  • , T. P. TourovaAffiliated withWinogradsky Institute of Microbiology, Russian Academy of Sciences

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

A novel strain, Z-7036, of anaerobic spore-forming bacteria was isolated from a cellulolytic consortium obtained from the bottom sediments of the low-mineralization soda lake Verkhnee Beloe (Buryatia). The cells of the new strain are short motile gram-positive rods, 1.1–3.0 × 0.25–0.4 μm. The organism is an aerotolerant anaerobe and obligate alkaliphile growing within the pH range of 7.5–9.7 with an optimum at pH 9.1. The strain is mesophilic and halotolerant and grows at NaCl concentrations from 0 to 50 g/l with an optimum at 20 g/l. Carbonates are required. The microorganism ferments peptone, yeast extract, trypticase, tryptone, Bacto Soytone, meat extract, Casamino acids, ornithine, arginine, threonine, and tryptophan. The strain hydrolyzes the bacterial preparations “Gaprin” and “Spirulina”. Acetate and formate are the major fermentation products. The strain reduces amorphous ferric hydroxide (AFH), EDTA-Fe(III), anthraquinone-2,6-disulfonate (quinone), S2O 3 2− , fumarate, and crotonate. Major fatty acids are C16:0, C16:1ω7c, iso-C17, iso-C15, and iso-C17:1. The DNA G+C content is 33.8 ± 0.5 mol %. According to the results of the 16S rRNA gene analysis, strain Z-7036 belongs to the genus Alkaliphilus within the cluster XI of low G+C gram-positive bacteria of the family Clostridiaceae. The novel strain is closely related to A. transvaalensis SAGM1T and A. crotonatoxidans B11-2T (93.3 and 93.9% 16S rRNA sequence identities, respectively). On the basis of the existing genotypic and phenotypic differences, we propose that strain Z-7036 should be classified as a novel species Alkaliphilus peptidofermentans sp. nov.

Key words

peptide fermentation soda lakes alkaliphiles anaerobes iron reduction Alkaliphilus