Abstract
Guanyl-specific ribonucleases from Bacillus intermedius and Bacillus pumilus are actively secreted under phosphate starvation by recombinant strains of Bacillus subtilis with native regulatory systems and by strains defective in some proteins of the Spo0A phosphorylation pathway. The level of expression of ribonuclease genes has been shown to increase approximately sixfold in recombinant strains with mutation in the spo0A gene and threefold in the spo0A/abrB mutants, as compared with native strains. These results demonstrate that the Spo0A protein regulates the production of ribonucleases and thus acts as a repressor, while the AbrB protein is an activator of expression of the genes encoding ribonucleases from Bacillus intermedius and Bacillus pumilus in Bacillus subtilis cells.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Struminskaya, N.K., Ivailovskii, V.L., Dement’ev, A.A., Moiseev, G.P., Fedosov, Yu.A., and Yakovlev, G.I., Extracellular ribonuclease in Bacillus subtilis, Biol. Nauki, 1992, no. 2, pp. 41–44.
Znamenskaya, L. V, Gabdrakhmanova, L.A., Chernokalskaya, E.V, Leshchinskaya, I.B., and Hartley, R.W., Phosphate regulation of biosynthesis of extracellular RNases of endospore-forming bacteria, FEBS Lett., 1995, vol. 375.
Znamenskaya, L.V., Vershinina, O.A., Vershinina, V.I., Krasnov, S.I., Kostrov, S.V., Akimkina, T.V., Leshchinskaya, I.B., and Hartley, R.V., Expression of Guanyl-Specific Ribonuclease Genes of Bacillus intermedius and Bacillus pumilus Is Regulated by the PhoP-PhoB Two-Component Signal-Transduction System of the PHO Regulon in Recombinant Bacillus subtilis Strains, Mikrobiologiya, 1999, vol. 68, no. 3, pp. 304–311 [Microbiology (Engl. Transl.), vol. 68, no. 3, pp. 258–264].
Vershinina, O.A. and Znamenskaya, L.V., The Pho Regulons of Bacteria, Mikrobiologiya, 2002, vol. 71, no. 5, pp. 581–595 [Microbiology (Engl. Transl.), vol. 68, no. 3, pp. 497–511].
Birkey, S.M., Wei, L., Zhang, X., Duggan, M., and Hulett, F.M., PHO Signal Trunsduction Network Reveals Direct Transcriptional Regulation of One Two-Component System by Another Two-Component Regulator: Bacillus subtilis PhoP Directly Regulates Production of ResD, Mol. Microbiol., 1998, vol. 30, pp. 943–953.
Klonirovanie DNK. Metody (DNA Cloning. Methods), Glover, D., Ed., Moscow: Mir, 1988.
Imanaka, T., Fujii, M., and Aiba, S., Isolation and characterization of antibiotic resistance plasmids from thermophilic bacilli and construction of deletion plasmids, J. Bacteriol., 1981, vol. 146, no. 3, pp. 1091–1097.
Jeffris, G.D., Holtman, W.F., and Guse, D., Rapid Method for Determining the Activity of Microorganisms on Nucleic Acids, J. Bacteriol., 1957, vol. 73, pp. 61–79.
Anfinsen, C.B., Redfield, R.R., Choate, W.I, Page, J., and Carrol, W.R., Studies of Gross Structure, Cross-Linkage and Terminal Sequences in Ribonuclease, J. Biol. Chem., 1957, vol. 207, pp. 201–210.
Burbulys, D., Trach, K.A., and Hoch, J.A., Initiation of Sporulation in Bacillus subtilis Is Controlled by a Multicomponent Phosphorelay, Cell, 1991, vol. 64, pp. 545–552.
Fujita, M., Gonzalez-Pastor, J.E., and Losick, R., High-and Low-Threshold Genes in the Spo0A Regulon of Bacillus subtilis, J. Bacteriol., 2005, vol. 187, no. 4, pp. 1357–1368.
Hulett, F.M., The Signal-Transduction Network for Pho Regulation in Bacillus subtilis, Mol. Microbiol., 1996, vol. 19, pp. 933–939.
Trach, K.A. and Hoch, J.A., Multisensory Activation of the Phosphorelay Initiating Sporulation in Bacillus subtilis: Identification and Sequence of the Protein Kinase of the Alternate Pathway, Mol. Microbiol., 1993, vol. 8, pp. 69–79.
Perego, M., A New Family of Aspartyl-Phosphate Phosphatases Targeting the Sporulation Transcription Factor Spo0A of Bacillus subtilis, J. Mol. Microbiol., 2001, vol. 42, pp. 133–144.
Perego, M., Cole, S.P., Burbulys, D., Trach, K., and Hoch, J.A., Characterization of the Gene for a Protein Kinase Which Phosphorylates the Sporulation-Regulatory Proteins Spo0A and Spo0F of Bacillus Subtilis, J. Bacteriol., 1989, vol. 171, no. 11, pp. 6187–6196.
Perego, M. and Hoch, J.A., Negative Regulation of Bacillus subtilis Sporulation by the Spo0E Gene Product, J. Bacteriol., 1991, vol. 173, no. 8, pp. 2514–2520.
Strauch, M.A. and Hoch, J.A., Transition-State Regulators: Sentinels of Bacillus subtilis Post-Exponential Gene Expression, Mol. Microbiol., 1993, vol. 7, pp. 337–342.
Phillips, Z.V. and Strauch, M.A., Bacillus subtilis Sporulation and Stationary Phase Gene Expression, CMLS Cell Mol. Life Sci., 2002, vol. 59, pp. 392–402.
Hamoen, L.W., Kausche, D., Marahiel, M.A., van Sinderen, D., Venema, G., and Serror, P., The Bacillus subtilis Transition State Regulator AbrB Binds To the −35 Promoter Region of comK, FEMS Microbiol. Letts., 2003, vol. 218, no. 2, pp. 299–304.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © V.V. Ul’yanova, V.I. Vershinina, M.A. Kharitonova, M.R. Sharipova, 2007, published in Mikrobiologiya, 2007, Vol. 76, No. 5, pp. 639–644.
Rights and permissions
About this article
Cite this article
Ul’yanova, V.V., Vershinina, V.I., Kharitonova, M.A. et al. The effect of Spo0A and AbrB proteins on expression of the genes of guanyl-specific ribonucleases from Bacillus intermedius and Bacillus pumilus in Bacillus subtilis recombinant strains. Microbiology 76, 563–568 (2007). https://doi.org/10.1134/S0026261707050086
Received:
Issue Date:
DOI: https://doi.org/10.1134/S0026261707050086