, Volume 75, Issue 6, pp 683-688

Effect of hydrogen concentration on the community structure of hydrogenotrophic methanogens studied by T-RELP analysis of 16S rRNA gene amplicons

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Abstract

Terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA genes was used to monitor the changes in the composition of the population of methanogens in enrichment cultures under high and low hydrogen concentrations. Hydrogen concentration was shown to determine the structure of a methanogenic community. High hydrogen concentration probably favors the hydrogen-and acetate-utilizing representatives of Methanosarcinaceae, while a more diverse methanogenic community is favored by low hydrogen concentrations.
Original Russian Text © A.I. Leybo, A.I. Netrusov, R. Conrad, 2006, published in Mikrobiologiya, 2006, Vol. 75, No. 6, pp. 786–791.