, Volume 75, Issue 6, pp 683–688

Effect of hydrogen concentration on the community structure of hydrogenotrophic methanogens studied by T-RELP analysis of 16S rRNA gene amplicons

Experimental Articles

DOI: 10.1134/S0026261706060105

Cite this article as:
Leybo, A.I., Netrusov, A.I. & Conrad, R. Microbiology (2006) 75: 683. doi:10.1134/S0026261706060105


Terminal restriction fragment length polymorphism (T-RFLP) analysis of 16S rRNA genes was used to monitor the changes in the composition of the population of methanogens in enrichment cultures under high and low hydrogen concentrations. Hydrogen concentration was shown to determine the structure of a methanogenic community. High hydrogen concentration probably favors the hydrogen-and acetate-utilizing representatives of Methanosarcinaceae, while a more diverse methanogenic community is favored by low hydrogen concentrations.

Key words

T-RFLP analysishydrogenotrophic methanogenssyntrophy

Copyright information

© Pleiades Publishing, Inc. 2006

Authors and Affiliations

  1. 1.Department of MicrobiologyMoscow State UniversityMoscowRussia
  2. 2.Department of BiogeochemistryMax Planck Institute of Terrestrial MicrobiologyMarburgGermany