Abstract
The work deals with studies on vasopressinergic neurons of hypothalamic supraoptic and paravenricular nuclei in the wild type mice and the neuronal nitric oxide synthase (nNOS) in the gene knockouted mice at a decrease of the brain catecholamine (CA) level caused by administration of the blocker of activity of tyrosine hydroxylase α-methyl-paratyrosine (α-MPT) and at the CA level decrease on the background of functional activity of the vasopressinergic neurons caused by dehydration of animals. There were analyzed changes in the number of neurons in the magnocellular hypothalamic nuclei expressing proapoptotic proteins caspase-8 and caspase-9, p53, and antiapoptotic protein Bcl-2. Disturbance of the CAergic innervation was shown to be a strong damaging factor leading to apoptosis of neurons regardless of the presence of nNOS in the cells. However, at disturbance of the CAergic innervation due to the 5-day mouse dehydration, no death of neurons by apoptosis was revealed. Thus, it is possible that functional activation prevents the hypothalamic vasopressinergic neurons from death at a decrease of the CA level in brain. The main difference of the nNOS gene knockouts is the absence of activation of the Bcl-2 expression under all used actions. This confirms our suggestion about interaction of CA and NO in triggering of expression of the antiapoptotic protein Bcl-2.
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Mayer, B. and Hemmens, B., Biosynthesis and Action of Nitric Oxide in Mammalian Cells, Trends Biochem. Sci., 1997, vol. 22, pp. 477–481.
Brüne, B., von Knethen, F., and Sandau, K.B., Nitric Oxide and Its Role in Apoptosis, Eur. J. Pharmacol., 1998, vol. 351, pp. 261–272.
Dawson, V.L., Dawson, T.M., Bartley, D.A., Uhl, G.R., and Snyder, S.H., Mechanisms of Nitric Oxide-Mediated Neurotoxicity in Primary Brain Cultures, J. Neurosci., 1993, vol. 13, pp. 2651–2661.
Lipton, S.A., Choi, Y.B., Pan, Z.H., Lei, S.Z., Chen, H.S., Sucher, N.J., Loscalzo, J., Singel, D.J., and Stamler, J.S., A Redox-Based Mechanism for the Neuroprotective and Neurodestructive Effects of Nitric Oxide and Related Nitroso-Compounds, Nature, 1993, vol. 364, pp. 626–632.
Liberatore, G.T., Jackson-Lewis, V., Vukosavic, S., Mandir, A.S., Vila, M., McAuliffe, W.G., Dawson, V.L., Dawson, T.M., and Przedborski, S., Inducible Nitric Oxide Synthase Stimulates Dopaminergic Neurodegeneration in the MPTP Model of Parkinson Disease, Nature Med., 1999, vol. 5, pp. 1403–1409.
Wu, D.Ch., Jackson-Lewis, V., Vila, M., Tieu, K., Teissmann, P., Vadseth, C., Choi, D.K., Ischiropoulos, H., and Przedborski, S., Blockade of Microglial Activation is Neuroprotective in the l-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Mouse Model of Parkinson Disease, J. Neurosci., 2002, vol. 22, pp. 1763–1771.
Ha, K.S., Kim, K.M., Kwon, Y.G., Bai, S.K., Nam, W.D., Yoo, Y.M., Kim, P.K., Chung, H.T., Billiar, T.R., and Kim, Y.M., Nitric Oxide Prevents 6-Hydroxydopamine-Induced Apoptosis in PC12 Cells through cGMP-Dependent PI3 Kinase/Akt Activation, FASEB J., 2003, vol. 17, pp. 1036–1047.
Chan, A.S., Ng, L.W., Poon, L.S., Chan, W.W., and Wong, Y.H., Dopaminergic and Adrenergic Toxicities on SK-N-MC Human Neuroblastoma Cells are Mediated through G Protein Signaling and Oxidative Stress, Apoptosis, 2007, vol. 12, pp. 167–179.
Alagarsamy, S., Phillips, M., Pappas, T., and Johnson, K.M., Dopamine Neurotoxicity in Cortical Neurons, Drug Alcohol. Depend., 1997, vol. 48, pp. 105–111.
Noh, J.S., Kim, E.Y., Kang, J.S., Kim, H.R., Oh, Y.J., and Gwag, B.J., Neurotoxic and Neuroprotective Actions of Catecholamines in Cortical Neurons, Exp. Neurol., 1999, vol. 159, pp. 217–224.
Purba, J.S., Hofman, M.A., and Swaab, D.F., Decreased Number of Oxytocin-Immunoreactive Neurons in the Paraventricular Nucleus of the Hypothalamus in Parkinson’s Disease, Neurology, 1994, vol. 44, pp. 84–89.
Sundquist, J., Forsling, M., Olsson, J., and Akerlundt, M., Cerebrospinal Fluid Arginine Vasopressin in Degenerative Disorders and Other Neurological Diseases, J. Neurol. Neurosurg. Psychiat., 1983, vol. 46, pp. 14–17.
Bugajski, J., Gadek-Michalska, A., Gld, R., and Borycz, J., Influence of Nitric Oxide Synthase Inhibitors on the Vasopressin-Induced Pituitary-Adrenocortical Activity, J. Physiol. Pharmacol., 1998, vol. 49, pp. 617–626.
Vacher, C.M., Hardin-Pouzet, H., Steinbusch, H.W., Calas, A., and De Vente, J., The Effects of Nitric Oxide on Magnocellular Neurons Could Involve Multiple Indirect Cyclic GMP-Dependent Pathways, Eur. J. Neurosci., 2003, vol. 17, pp. 455–466.
Yamova, L., Atochin, D., Glazova, M., Chernigovskaya, E., and Huang, P., Role of Neuronal Nitric Oxide in the Regulation of Vasopressin Expression and Release in Response to Inhibition of Catecholamine Synthesis and Dehydration, Neurosci. Lett., 2007, vol. 426, pp. 160–165.
Taranukhin, A.G., Glazova, M.V., Evteeva, S.E., Iamova, L.A., and Chernigovskaya, E.V., Catecholamines and Nitric Oxide Participation in the Regulation of Apoptosis in Nonapeptidergic Neurons in the Rat Hypothalamus, Zh. Evol. Biokhim. Fiziol., 2002, vol. 38, pp. 615–619.
Widerlov, E., Dose-Dependent Pharmacokinetics of α-Methyl-p-Tyrosine (α-MT) and Comparison of Catecholamine Turnover Rates after Two Doses of α-MT, J. Neural Transmis., 1979, vol. 44, pp. 145–158.
Kuida, K., Caspase-9, Int. J. Biochem. Cell Biol., 2000, vol. 32, no. 2, pp. 121–124.
Li, P., Nijhawan, D., Budihardjo, I., Srinivasula, S.M., Ahmad, M., Alnemri, E.S., and Wang, X., Cytochrome c and dATP-Dependent Formation of Apaf-1/Caspase-9 Complex Initiates an Apoptotic Protease Cascade, Cell, 1997, vol. 91, pp. 479–489.
Morris, E.J., Keramaris, E., Rideout, H.J., Slack, R.S., Dyson, N.J., Stefanis, L., and Park, D.S., Cyclin-Dependent Kinase and p53 Pathways are Activated Independently and Mediate Bax Activation in Neurons after DNA Damage, J. Neurosci., 2001, vol. 21, pp. 5017–5026.
Moroni, M.C., Hickman, E.S., Lazzerini Denchi, E., Caprara, G., Colli, E., Cecconi, F., Müller, H., and Helin, K., Apaf-1 Is a Transcriptional Target for E2F and p53, Natl. Cell. Biol. 2001, vol. 3, pp. 552–558.
Bargonetti, J. and Manfredi, J.J., Multiple Roles of the Tumor Suppressor p53, Curr. Opin. Oncol., 2002, vol. 14, pp. 86–91.
Cregan, S.P., Fortin, A., MacLaurin, J.G., Callaghan, S.M., Cecconi, F., Yu, S.W., Dawson, T.M., Dawson, V.L., Park, D.S., Kroemer, G., and Slack, R.S., Apoptosis-Inducing Factor Is Involved in the Regulation of Caspase-Independent Neuronal Cell Death, J. Cell. Biol., 2002, vol. 158, pp. 507–517.
Stennicke, H.R. and Salvesen, G.S., Properties of the Caspases, Biochim. Biophys. Acta, 1998, vol. 1387, pp. 17–31.
Chernigovskaya, E.V., Taranukhin, A.G., Glazova, M.V., Yamova, L.A., and Fedorov, L.M., Apoptotic Signaling Proteins: Possible Participation in the Regulation of Vasopressin and Cat echolamines Biosynthesis in the Hypothalamus, Histochem. Cell. Biol., 2005, vol. 124, pp. 523–533.
Chernigovskaya, E.V., Nikitina, L.S., Dorofeeva, N.A., and Glazova, M.V., Effects of Selective Bcl-2 Inhibitor HA14-1 Treatments on Functional Activity of Magnocellular Vasopressinergic Neurons of Rat Hypothalamus, Neurosci. Lett., 2008, vol. 437, pp. 59–64.
Kova, K.J., Fo, A., and Sawchenko, P.E., Glucocorticoid Negative Feedback Selectively Targets Vasopressin Transcription in Parvocellular Neurosecretory Neurons, J. Neurosci., 2000, vol. 20, pp. 3843–3852.
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Original Russian Text © E. V. Chernigovskaya, L. A. Yamova, D. Atochin, P. Huang, M. V. Glazova, 2011, published in Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, 2011, Vol. 47, No. 3, pp. 232–238.
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Chernigovskaya, E.V., Yamova, L.A., Atochin, D. et al. Interaction of neuronal NOS and catecholamines in regulation of expression of proteins of apoptosis by vasopressinergic hypothalamic neurons. J Evol Biochem Phys 47, 275–283 (2011). https://doi.org/10.1134/S0022093011030078
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DOI: https://doi.org/10.1134/S0022093011030078