Article

Biochemistry (Moscow)

, Volume 77, Issue 11, pp 1315-1325

First online:

Molecular cloning, isolation, and properties of chaperone Skp from Yersinia pseudotuberculosis

  • E. V. SidorinAffiliated withElyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences Email author 
  • , N. M. TishchenkoAffiliated withElyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences
  • , V. A. KhomenkoAffiliated withElyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences
  • , M. P. IsaevaAffiliated withElyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences
  • , P. S. DmitrenokAffiliated withElyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences
  • , N. Yu. KimAffiliated withElyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences
  • , G. N. LikhatskayaAffiliated withElyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences
  • , T. F. Solov’evaAffiliated withElyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of the Russian Academy of Sciences

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Abstract

The skp gene of Yersinia pseudotuberculosis was expressed without its signal sequence in Escherichia coli BL21(DE3) cells. The recombinant protein Skp accumulated in soluble form in the cytoplasm of the producer strain. The protein was isolated and characterized: the molecular weight, isoelectric point, N-terminal amino acid sequence (20 amino acid residues), and the content of the secondary structure elements were determined. Using cross-linking stabilization and high-mass MALDI-TOF mass spectrometric analysis, it was found that rSkp forms a stable homotrimer in solution and interacts with human IgG. Three-dimensional models of the Skp trimer and its complexes with Fc- and Fab-fragments of human IgG1 were constructed by computer modeling.

Key words

chaperone Skp Yersinia pseudotuberculosis immunoglobulin G cross-linking stabilization MALDI-TOF mass spectrometry computer modeling protein-protein interactions