Biochemistry (Moscow)

, Volume 77, Issue 1, pp 78–86

Effect of amiodarone on thermotolerance and Hsp104p synthesis in the yeast Saccharomyces cerevisiae

  • I. V. Fedoseeva
  • D. V. Pjatricas
  • N. N. Varakina
  • T. M. Rusaleva
  • A. V. Stepanov
  • E. G. Rikhvanov
  • G. B. Borovskii
  • V. K. Voinikov
Article

DOI: 10.1134/S0006297912010099

Cite this article as:
Fedoseeva, I.V., Pjatricas, D.V., Varakina, N.N. et al. Biochemistry Moscow (2012) 77: 78. doi:10.1134/S0006297912010099

Abstract

Amiodarone (AMD) is known to induce a transient increase in cytosolic Ca2+ level in cells of the yeast Saccharomyces cerevisiae. In the present study the effect of AMD on the thermotolerance and Hsp104p synthesis of the yeast was studied. AMD induced Hsp104p synthesis and increased survival of the yeast after a severe heat shock (50°C). The development of thermotolerance to a considerable extent depended on the presence of Hsp104p. The same effect was achieved by treatment with the classical uncoupler CCCP, which is also known to increase the cytosolic Ca2+ level. It is supposed that the change in intracellular Ca2+ concentration plays an important role in activation of the HSP104 gene expression and in increasing the thermotolerance of the yeast. The possible link between mitochondrial activity and calcium homeostasis is discussed.

Key words

amiodaroneSaccharomyces cerevisiaethermotoleranceHsp104pmitochondrion

Abbreviations

AMD

amiodarone (2-butyl-3-(3,5-diiodo-4-diethylaminoethoxybenzoyl)-benzofuran)

CCCP

carbonyl cyanide 3-chlorophehylhydrazone

FDA

fluorescein diacetate

Hsf

heat shock factor

Hsp

heat shock protein

mtΔψ

transmembrane potential on the inner mitochondrial membrane

PCD

programmed cell death

PI

propidium iodide

ROS

reactive oxygen species

Copyright information

© Pleiades Publishing, Ltd. 2012

Authors and Affiliations

  • I. V. Fedoseeva
    • 1
  • D. V. Pjatricas
    • 1
  • N. N. Varakina
    • 1
  • T. M. Rusaleva
    • 1
  • A. V. Stepanov
    • 1
  • E. G. Rikhvanov
    • 1
  • G. B. Borovskii
    • 1
  • V. K. Voinikov
    • 1
  1. 1.Siberian Institute of Plant Physiology and BiochemistrySiberian Division of the Russian Academy of SciencesIrkutskRussia