Biochemistry (Moscow)

, Volume 76, Issue 7, pp 755–760

Structural diversity of the core oligosaccharide domain of Pseudomonas aeruginosa lipopolysaccharide

Review

DOI: 10.1134/S0006297911070054

Cite this article as:
Kocincova, D. & Lam, J.S. Biochemistry Moscow (2011) 76: 755. doi:10.1134/S0006297911070054

Abstract

Pseudomonas aeruginosa is a Gram-negative bacterium that is ubiquitous in the environment and generally considered to be a saprophyte, but it is also an important opportunistic human pathogen. Pseudomonas aeruginosa elaborates a variety of virulence factors, one of which is lipopolysaccharide (LPS). LPS of P. aeruginosa is composed of three distinct regions: lipid A, core oligosaccharide (OS), and the long-chain O antigen. The core OS of P. aeruginosa is composed of L-glycero-D-manno-heptose, 3-deoxy-D-manno-oct-2-ulosonic acid, D-galactosamine, D-glucose, and L-rhamnose. Noncarbohydrate substituents are also found in the core OS including phosphate, 2-aminoethyl (di)phosphate, acetyl, alanyl and carbamoyl groups. Pseudomonas aeruginosa simultaneously synthesizes two core glycoforms, namely, capped and uncapped core. The capped core is covalently attached to an O antigen, whereas the uncapped core is devoid of O antigen. Although the core of P. aeruginosa LPS is relatively conserved, strain-to-strain variability of its structure exists. This includes phosphorylation pattern, the level of O-acetylation, and the presence or absence of a fourth glucose residue at the distal end of the uncapped core. A number of studies have been reported on the structures of unique truncated core OS with unusual modifications. This mini-review summarizes the diversity of P. aeruginosa complete and truncated core OS structures published over the past fifteen years

Key words

lipopolysaccharide core oligosaccharide Pseudomonas aeruginosa 

Abbreviations

GalN

galactosamine

Hep

L-glycero-D-manno-heptose

IATS

International Antigenic Typing Scheme

Kdo

3-deoxy-D-manno-oct-2-ulosonic acid

LPS

lipopolysaccharide

MS

mass spectrometry

OS

oligosaccha-ride

P

phosphate

Rha

rhamnose

Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  1. 1.Department of Molecular and Cellular BiologyUniversity of GuelphGuelphCanada