Biochemistry (Moscow)

, 76:497

Fluorescence correlation spectroscopy in biology, chemistry, and medicine

  • I. V. Perevoshchikova
  • E. A. Kotova
  • Y. N. Antonenko
Review

DOI: 10.1134/S0006297911050014

Cite this article as:
Perevoshchikova, I.V., Kotova, E.A. & Antonenko, Y.N. Biochemistry Moscow (2011) 76: 497. doi:10.1134/S0006297911050014

Abstract

This review describes the method of fluorescence correlation spectroscopy (FCS) and its applications. FCS is used for investigating processes associated with changes in the mobility of molecules and complexes and allows researchers to study aggregation of particles, binding of fluorescent molecules with supramolecular complexes, lipid vesicles, etc. The size of objects under study varies from a few angstroms for dye molecules to hundreds of nanometers for nanoparticles. The described applications of FCS comprise various fields from simple chemical systems of solution/micelle to sophisticated regulations on the level of living cells. Both the methodical bases and the theoretical principles of FCS are simple and available. The present review is concentrated preferentially on FCS applications for studies on artificial and natural membranes. At present, in contrast to the related approach of dynamic light scattering, FCS is poorly known in Russia, although it is widely employed in laboratories of other countries. The goal of this review is to promote the development of FCS in Russia so that this technique could occupy the position it deserves in modern Russian science.

Key words

fluorescence fluctuationscorrelation spectroscopydiffusionaggregationbindingnanoparticlesbiopolymersvesiclesligandsreceptorsconformational changesnucleic acid and protein folding

Abbreviations

DNP

2,4-dinitrophenol

FCS

fluorescence correlation spectroscopy

G(τ)

autocorrelation function

PCH

photon counting histogram

TMRE

tetramethylrhodamine ethyl ester

τd

diffusion time

Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  • I. V. Perevoshchikova
    • 1
    • 2
  • E. A. Kotova
    • 1
  • Y. N. Antonenko
    • 1
  1. 1.Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia
  2. 2.Department of Biomedical SciencesUniversity of Veterinary MedicineViennaAustria