Biochemistry (Moscow)

, 73:420

Inhibition of catalase by aminotriazole in vivo results in reduction of glucose-6-phosphate dehydrogenase activity in Saccharomyces cerevisiae cells

Authors

  • M. Bayliak
    • Department of BiochemistryVassyl Stefanyk Precarpathian National University
  • D. Gospodaryov
    • Department of BiochemistryVassyl Stefanyk Precarpathian National University
  • H. Semchyshyn
    • Department of BiochemistryVassyl Stefanyk Precarpathian National University
    • Department of BiochemistryVassyl Stefanyk Precarpathian National University
Article

DOI: 10.1134/S0006297908040068

Cite this article as:
Bayliak, M., Gospodaryov, D., Semchyshyn, H. et al. Biochemistry Moscow (2008) 73: 420. doi:10.1134/S0006297908040068
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Abstract

The inhibitor of catalase 3-amino-1,2,4-triazole (AMT) was used to study the physiological role of catalase in the yeast Saccharomyces cerevisiae under starvation. It was shown that AMT at the concentration of 10 mM did not affect the growth of the yeast. In vivo and in vitro the degree of catalase inhibition by AMT was concentration- and time-dependent. Peroxisomal catalase in bakers’ yeast was more sensitive to AMT than the cytosolic one. In vivo inhibition of catalase by AMT in S. cerevisiae caused a simultaneous decrease in glucose-6-phosphate dehydrogenase activity and an increase in glutathione reductase activity. At the same time, the level of protein carbonyls, a marker of oxidative modification, was not affected. Possible mechanisms compensating the negative effects caused by AMT inhibition of catalase are discussed.

Key words

Saccharomyces cerevisiae3-amino-1,2,4-triazolecatalaseglucose-6-phosphate dehydrogenase

Abbreviations

AMT

3-amino-1,2,4-triazole

CP

protein carbonyls

G6PDH

glucose-6-phosphate dehydrogenase

GR

glutathione reductase

ICDH

isocitrate dehydrogenase

LDH

lactate dehydrogenase

PMSF

phenylmethylsulfonyl fluoride

ROS

reactive oxygen species

SOD

superoxide dismutase

TEMED

N,N,N′,N′-tetramethylethylenediamine

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© MAIK Nauka 2008