Biochemistry (Moscow)

, Volume 72, Issue 7, pp 683–697

Genetically encoded intracellular sensors based on fluorescent proteins

Review

DOI: 10.1134/S0006297907070012

Cite this article as:
Souslova, E.A. & Chudakov, D.M. Biochemistry Moscow (2007) 72: 683. doi:10.1134/S0006297907070012

Abstract

Green fluorescent protein from Aequorea victoria and its many homologs are now widely used in basic and applied research. These genetically encoded fluorescent markers can detect localization of cell proteins and organelles in living cells and also cells and tissues in living organisms. Unique instruments and methods for studies of molecular biology of a cell and high throughput drug screenings are based on fluorescent proteins. This review deals with the most intensively evolving directions in this field, the development of genetically encoded sensors. Changes in their spectral properties are used for monitoring of cell enzyme activities or changes in concentrations of particular molecules.

Key words

genetically encoded sensors fluorescent proteins mutant GFP variants FRET 

Abbreviations

BFP

blue fluorescent protein

CaM

calmodulin

CFP

cyan fluorescent protein

EGFP

enhanced green fluorescent protein

EGFR

epidermal growth factor receptor

FLIM

fluorescence lifetime imaging microscopy

FP

fluorescent protein

FRET

Forster resonance energy transfer

GES

genetically encoded sensors

GFP

green fluorescent protein

GKI

protein kinase G

MLCK

myosin light chain kinase

M13

calmodulin-binding fragment of MLCK

PDE

phosphodiesterase

PKA

protein kinase A

TnC

troponin C

YC

Yellow Cameleons

YFP

yellow fluorescent protein

Copyright information

© Pleiades Publishing, Ltd. 2007

Authors and Affiliations

  1. 1.Shemyakin-Ovchinnikov Institute of Bioorganic ChemistryRussian Academy of SciencesMoscowRussia

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