Article

Biochemistry (Moscow)

, Volume 71, Issue 9, pp 1021-1026

First online:

Hepatitis C virus RNA-dependent RNA polymerase: Study on the inhibition mechanism by pyrogallol derivatives

  • M. V. KozlovAffiliated withEngelhardt Institute of Molecular Biology, Russian Academy of Sciences Email author 
  • , K. M. PolyakovAffiliated withEngelhardt Institute of Molecular Biology, Russian Academy of Sciences
  • , A. V. IvanovAffiliated withEngelhardt Institute of Molecular Biology, Russian Academy of Sciences
  • , S. E. FilippovaAffiliated withEngelhardt Institute of Molecular Biology, Russian Academy of Sciences
  • , A. O. KuzyakinAffiliated withEngelhardt Institute of Molecular Biology, Russian Academy of Sciences
  • , V. L. TunitskayaAffiliated withEngelhardt Institute of Molecular Biology, Russian Academy of Sciences
  • , S. N. KochetkovAffiliated withEngelhardt Institute of Molecular Biology, Russian Academy of Sciences

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Abstract

Pyrogallol reversibly and noncompetitively inhibits the activity of the hepatitis C RNA-dependent RNA polyme rase. Based on molecular modeling of the inhibitor binding in the active site of the enzyme, the inhibition was suggested to be realized via chelation of two magnesium cations involved in the catalysis at the stage of the phosphoryl residue transfer. The proposed model allowed us to purposefully synthesize new derivatives with higher inhibitory capacity.

Key words

hepatitis C virus RNA-dependent RNA polymerase pyrogallol derivatives inhibition mechanism molecular modeling