Biochemistry (Moscow)

, Volume 71, Issue 8, pp 846–850

New viral vector for efficient production of target proteins in plants

  • T. V. Komarova
  • M. V. Skulachev
  • A. S. Zvereva
  • A. M. Schwartz
  • Yu. L. Dorokhov
  • J. G. Atabekov
Accelerated Publication

DOI: 10.1134/S0006297906080049

Cite this article as:
Komarova, T.V., Skulachev, M.V., Zvereva, A.S. et al. Biochemistry (Moscow) (2006) 71: 846. doi:10.1134/S0006297906080049

Abstract

A new potato virus X (PVX)-based viral vector for superproduction of target proteins in plants has been constructed. The triple gene block and coat protein gene of PVX were substituted by green fluorescent protein. This reduced viral vector was delivered into plant cells by agroinjection (injection of Agrobacterium tumefaciens cells, carrying viral vector cDNA within T-DNA, into plant leaves), and this approach allowed to dramatically reduce the size of the vector genome. The novel vector can be used for production of different proteins including pharmaceuticals in plants.

Key words

potato virus Xviral vectoragroinjectionprotein superexpression

Abbrevations

CP

coat protein

crTMV

crucifer infecting tobamovirus

GFP

green fluorescent protein

MP

movement protein

PVA

potato virus A

PVX

potato virus X

RdRp

RNA-dependent RNA-polymerase

SGP

subgenomic promoter

sgRNA

subgenomic RNA

TBSV

tomato bushy stunt virus

T-DNA

a fragment of Ti (tumor inducing) plasmid which is transferred into plant cell nucleus

TGB

triple gene block

TMV

tobacco mosaic virus

3′-UTR

3′-untranslated region

VIGS

virus-induced gene silencing

Copyright information

© Pleiades Publishing, Inc. 2006

Authors and Affiliations

  • T. V. Komarova
    • 1
  • M. V. Skulachev
    • 1
  • A. S. Zvereva
    • 1
  • A. M. Schwartz
    • 1
  • Yu. L. Dorokhov
    • 1
  • J. G. Atabekov
    • 1
  1. 1.Faculty of Biology and Belozersky Institute of Physico-Chemical BiologyLomonosov Moscow State UniversityMoscowRussia