Applied Biochemistry and Microbiology

, Volume 47, Issue 4, pp 381–385

Heterogenous expression of poly-γ-glutamic acid synthetase complex gene of Bacillus licheniformis WBL-3

Article

DOI: 10.1134/S0003683811040193

Cite this article as:
Wang, N., Yang, G., Che, C. et al. Appl Biochem Microbiol (2011) 47: 381. doi:10.1134/S0003683811040193

Abstract

Bacillus licheniformis WBL-3, one of poly-γ-glutamic acid (γ-PGA) producers, depends on the existence of glutamate in the medium. In this paper, γ-PGA synthetase complex gene (pgsBCA) was cloned from Bacillus licheniformis WBL-3. pgsBCA gene of B. licheniformis WBL-3 was highly homologous with pgs-BCA gene of B. licheniformis 14580. The similarity was 97%, but the similarity of pgsBCA gene between B. licheniformis WBL-3 and Bacillus subtilis IFO3336 was only 74%. However, when pgsBCA was expressed in Escherichia coli, the E. coli clone produced γ-PGA extracellularly. The yield of γ-PGA was 8.624 g/l. This result infers that B. licheniformis and B. subtilis has the similar γ-PGA biosynthesis mechanism, namely, glutamic acid is catalyzed by an ATP-dependent amide ligase to synthesize γ-PGA.

Copyright information

© Pleiades Publishing, Ltd. 2011

Authors and Affiliations

  1. 1.College of Life ScienceQufu Normal UniversityQufuR.R. China