Applied Biochemistry and Microbiology

, Volume 45, Issue 5, pp 498–502

Metabolism characteristics of Chelativorans oligotrophicus by two-phase growth on the mixture of EDTA and glucose

  • E. N. Kaparullina
  • N. V. Doronina
  • L. V. Trilisenko
  • V. M. Vagabov
  • Yu. A. Trotsenko
Article

DOI: 10.1134/S000368380905007X

Cite this article as:
Kaparullina, E.N., Doronina, N.V., Trilisenko, L.V. et al. Appl Biochem Microbiol (2009) 45: 498. doi:10.1134/S000368380905007X

Abstract

The obligate destructor of ethylene diamine tetraacetate—a culture of Chelativorans oligotrophicus LPM-4—did not grow on a medium with glucose, but it was good to use it under cultivation on a mixture with EDTA after considerable decrease of the EDTA concentration in the medium (two-phase growth). Strong inhibition of hexokinase and glucose 6-phosphate dehydrogenase in cell exracts 4 mM EDTA was revealed. Using EDTA, cells accumulated polyphosphates whose rate decreased during glucose utilization phase. High activities of polyphosphate biosynthesis ferments (adenylat kinase and polyphosphate kinase) were distinguished during the first phase of the cultivation; considerable decrease of them and increase of polyphosphate glucokinase were found during the second phase of the cultivation. This points to the possible participating of polyphosphates in glucose metabolism as a supplementary energy source.

Copyright information

© Pleiades Publishing, Ltd. 2009

Authors and Affiliations

  • E. N. Kaparullina
    • 1
  • N. V. Doronina
    • 1
  • L. V. Trilisenko
    • 1
  • V. M. Vagabov
    • 1
  • Yu. A. Trotsenko
    • 1
  1. 1.Skryabin Institute of Biochemistry and Physiology of MicroorganismsRussian Academy of SciencesPushchino, Moscow oblastRussia