Applied Biochemistry and Microbiology

, Volume 43, Issue 6, pp 655–660

Inhibition of lipase activity by low-molecular-weight chitosan

  • E. S. Ostanina
  • V. P. Varlamov
  • G. I. Yakovlev
Article

DOI: 10.1134/S0003683807060154

Cite this article as:
Ostanina, E.S., Varlamov, V.P. & Yakovlev, G.I. Appl Biochem Microbiol (2007) 43: 655. doi:10.1134/S0003683807060154

Abstract

Inhibition of enzymatic activity of lipase (EC 3.1.1.3) from the fungus Candida rugosa and wheat (Triticum aestivum L.) germ by low-molecular-weight chitosan with an average molecular weight of 5.7 kDa in reactions of p-nitrophenyl palmitate cleavage was studied. Preincubation of lipases with chitosan, prior to addition of the substrate to solution, showed that equilibrium during the lipase-inhibitor complex formation was reached within 30 min. The inhibition constants for C. rugosa lipase and wheat germ lipase were 1.4 and 0.9 mM, respectively. The contribution of electrostatic interactions to the complex formation between chitosan and lipases is insignificant.

Copyright information

© Pleiades Publishing, Inc. 2007

Authors and Affiliations

  • E. S. Ostanina
    • 1
  • V. P. Varlamov
    • 1
  • G. I. Yakovlev
    • 2
  1. 1.Bioengineering CenterRussian Academy of SciencesMoscowRussia
  2. 2.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia

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