, Volume 43, Issue 5, pp 550-555

Use of soybean peroxidase for the enzyme immunoassay of sulfamethoxipyridazine in milk

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Abstract

An enzyme immunoassay with colorimetric detection of sulfamethoxipyridazine (SMP), the most widely used sulfamide, was developed with the soybean anionic peroxidase as an enzyme marker. The range of SMP detection is 1.3–63.0 ng/ml with a detection limit of 0.4 ng/ml. The root square deviation of detection results did not exceed 6%. It was demonstrated that 0.15% casein added to the working buffer prevented the effect of the milk matrix on the detection. The results obtained demonstrate that the assay developed is promising, displaying a sensitivity that exceeds the maximum permissible concentration of sulfamides in milk (100 μg/l) by several orders of magnitude.

Original Russian Text © A.N. Berlina, A.V. Zherdev, B.B. Dzantiev, I.Yu. Sakharov, 2007, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2007, Vol. 43, No. 5, pp. 614–620.