Abstract
Crystallization of bacteriorhodopsin (bR) in the lipidic cubic phase using n-octyl-β-D-glucoside (OG) and its more stable and inexpensive analogue n-octyl-β-D-thioglucoside (OTG) was comparatively analyzed [1]. It was shown that bacteriorhodopsin is efficiently crystallized in OTG in the same detergent concentration range as in OG. However, x-ray diffraction analysis shows that bR crystals in OG are characterized by a better resolution (1.35 Å) than bR crystals in OTG (1.45 Å).
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References
T. Tsuchiya and S. Saito, J. Biochem. 96, 1593 (1984).
http://www.mpibp-frankfurt.mpg.de/michel/public/memprotstruct.html
A. McPherson, Crystallization of Biological Macromolecules (Cold Spring Harbor Laboratory, New York, 1998), p. 252.
E. Landau and J. Rosenbusch, Proc. Natl. Acad. Sci. USA 93, 14532 (1996).
I. Gordeliy, J. Labahn, R. Moukhametzianov, et al., Nature 419, 484 (2002).
R. Moukhametzianov, J. P. Klare, R. Efremov, et al., Nature 440, 115 (2006).
M. Caffrey, J. Struct. Biol. 142, 108 (2003).
J. K. Lanyi, J. Mol. Microbiol. Biotechnol. 12, 210 (2007).
M. P. Krebs and T. A. Isenbarger, Biochim. Biophys. Acta 1460, 15 (2000).
D. Oesterhelt and W. Stoeckenius, Methods. Enzymol. 31, 667 (1974).
I. Gordeliy, R. Schlesinger, R. Efremov, et al., Methods Mol. Biol. 228, 305 (2003).
A. G. W. Leslie, Acta Crystallogr. D Biol. Cryst. 62, 48 (2006).
Collaborat. Computational Project No. 4, Acta Crystallogr. D 50, 760 (1994).
S. Saito and T. Tsuchiya, Biochem. J. 222, 829 (1984).
B. Lorber, J. Bishop, and L. J. DeLucas, Biochim. Biophys. Acta 1023, 254 (1990).
C. Sauter, J. D. Ng, B. Lorber, et al., J. Crystal Growth 196, 365 (1999).
J. P. Anderson, G. Basi, M. T. Doan, et al. (Elan Pharmaceuticals, Inc.), β-Secretase Enzyme Compositions and Methods, US Patent No. 7067271 (2006); http://www.freepatentsonline.com/7067271.html
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Original Russian Text © E.S. Moiseeva, A.B. Reshetnyak, V.I. Borshchevskiy, C. Baeken, G. Buldt, V.I. Gordeliy, 2009, published in Poverkhnost’. Rentgenovskie, Sinkhrotronnye i Neitronnye Issledovaniya, No. 1, pp. 34–37.
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Moiseeva, E.S., Reshetnyak, A.B., Borshchevskiy, V.I. et al. Comparative analysis of the quality of membrane protein bacteriorhodopsin crystals during crystallization in octylglucoside and octylthioglucoside. J. Surf. Investig. 3, 29–32 (2009). https://doi.org/10.1134/S1027451009010054
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DOI: https://doi.org/10.1134/S1027451009010054