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Structure of DNA complexes with chromosomal protein HMGB1 and histone H1 in the presence of manganese ions: 2. Vibrational circular dichroism spectroscopy

  • Structural and Functional Analysis of Biopolymers and Their Complexes
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Abstract

DNA complexes with nonhistone HMGB1 chromatin protein and histone H1 in the presence of manganese ions were studied using methods of absorption and circular dichroism spectroscopy in the infrared region. It was demonstrated that the method provides good results, even for solutions that contain large particles, which cause scattering in UV region. It was determined that manganese ions in the complex are able to coordinate not only to different chemical groups in DNA, but also to dicarboxylic acid residues of the HMGB1 protein, which stimulates DNA condensation and slightly weakens DNA-protein interactions in the complex.

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Authors and Affiliations

  1. Department of Physics, St. Petersburg State University, St. Petersburg, Staryi Pitergof, 198504, Russia

    A. M. Polyanichko & V. I. Vorob’ev

  2. Institute of Cytology, Russian Academy of Sciences, St. Petersburg, 194064, Russia

    A. M. Polyanichko, V. I. Vorob’ev & E. V. Chikhirzhina

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  1. A. M. Polyanichko
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  2. V. I. Vorob’ev
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  3. E. V. Chikhirzhina
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Correspondence to A. M. Polyanichko.

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Original Russian Text © A.M. Polyanichko, V. I. Vorob’ev, E.V. Chikhirzhina, 2013, published in Molekulyarnaya Biologiya, 2013, Vol. 47, No. 2, pp. 338–346.

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Polyanichko, A.M., Vorob’ev, V.I. & Chikhirzhina, E.V. Structure of DNA complexes with chromosomal protein HMGB1 and histone H1 in the presence of manganese ions: 2. Vibrational circular dichroism spectroscopy. Mol Biol 47, 299–306 (2013). https://doi.org/10.1134/S0026893313020118

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