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Structural diversity of the core oligosaccharide domain of Pseudomonas aeruginosa lipopolysaccharide

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Abstract

Pseudomonas aeruginosa is a Gram-negative bacterium that is ubiquitous in the environment and generally considered to be a saprophyte, but it is also an important opportunistic human pathogen. Pseudomonas aeruginosa elaborates a variety of virulence factors, one of which is lipopolysaccharide (LPS). LPS of P. aeruginosa is composed of three distinct regions: lipid A, core oligosaccharide (OS), and the long-chain O antigen. The core OS of P. aeruginosa is composed of L-glycero-D-manno-heptose, 3-deoxy-D-manno-oct-2-ulosonic acid, D-galactosamine, D-glucose, and L-rhamnose. Noncarbohydrate substituents are also found in the core OS including phosphate, 2-aminoethyl (di)phosphate, acetyl, alanyl and carbamoyl groups. Pseudomonas aeruginosa simultaneously synthesizes two core glycoforms, namely, capped and uncapped core. The capped core is covalently attached to an O antigen, whereas the uncapped core is devoid of O antigen. Although the core of P. aeruginosa LPS is relatively conserved, strain-to-strain variability of its structure exists. This includes phosphorylation pattern, the level of O-acetylation, and the presence or absence of a fourth glucose residue at the distal end of the uncapped core. A number of studies have been reported on the structures of unique truncated core OS with unusual modifications. This mini-review summarizes the diversity of P. aeruginosa complete and truncated core OS structures published over the past fifteen years

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Abbreviations

GalN:

galactosamine

Hep:

L-glycero-D-manno-heptose

IATS:

International Antigenic Typing Scheme

Kdo:

3-deoxy-D-manno-oct-2-ulosonic acid

LPS:

lipopolysaccharide

MS:

mass spectrometry

OS:

oligosaccha-ride

P:

phosphate

Rha:

rhamnose

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Correspondence to J. S. Lam.

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Published in Russian in Biokhimiya, 2011, Vol. 76, No. 7, pp. 925–932.

The authors dedicate this review to Dr. Malcolm Perry in our admiration of the impact that he has made to the field of complex carbohydrate research during his long and fruitful career at the National Research Council of Canada.

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Kocincova, D., Lam, J.S. Structural diversity of the core oligosaccharide domain of Pseudomonas aeruginosa lipopolysaccharide. Biochemistry Moscow 76, 755–760 (2011). https://doi.org/10.1134/S0006297911070054

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  • DOI: https://doi.org/10.1134/S0006297911070054

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