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Nicking endonucleases

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Abstract

Nicking endonucleases are a new type of enzymes. Like restriction endonucleases, they recognize short specific DNA sequence and cleave DNA at a fixed position relatively to the recognition sequence. However, unlike restriction endonucleases, nicking endonucleases cleave only one predetermined DNA strand. Until recently, nicking endonucleases were suggested to be naturally mutated restriction endonucleases which had lost their ability to dimerize and as a result the ability to cleave the second strand. We have shown that nicking endonucleases are one of the subunits of heterodimeric restriction endonucleases. Mechanisms used by various restriction endonucleases for double-stranded cleavage, designing of artificial nicking endonucleases on the basis of restriction endonucleases, and application of nicking endonucleases in molecular biology are reviewed.

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Abbreviations

a.a.:

amino acid residue

bp:

base pairs

dNTP:

mixture of deoxynucleoside triphosphates

MTase:

DNA methyltransferase

NICKase:

nicking endonuclease

ORF:

open reading frame

PCR:

polymerase chain reaction

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Author information

Authors and Affiliations

  1. Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia

    L. A. Zheleznaya, G. S. Kachalova, R. I. Artyukh, A. K. Yunusova & T. A. Perevyazova

  2. Max Plank Research Unit for Structural Molecular Biology, Hamburg, Germany

    G. S. Kachalova

  3. Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Moscow Region, Russia

    N. I. Matvienko

Authors
  1. L. A. Zheleznaya
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  2. G. S. Kachalova
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  3. R. I. Artyukh
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  4. A. K. Yunusova
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  5. T. A. Perevyazova
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  6. N. I. Matvienko
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Corresponding author

Correspondence to L. A. Zheleznaya.

Additional information

Original Russian Text © L. A. Zheleznaya, G. S. Kachalova, R. I. Artyukh, A. K. Yunusova, T. A. Perevyazova, N. I. Matvienko, 2009, published in Uspekhi Biologicheskoi Khimii, 2009, Vol. 49, pp. 107–128.

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Zheleznaya, L.A., Kachalova, G.S., Artyukh, R.I. et al. Nicking endonucleases. Biochemistry Moscow 74, 1457–1466 (2009). https://doi.org/10.1134/S0006297909130033

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  • DOI: https://doi.org/10.1134/S0006297909130033

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