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Analysis of proteins interacting with TRIP8b adapter

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Abstract

Calcium-independent receptor of latrotoxin (CIRL) is an orphan heptahelical receptor implicated in regulation of exocytosis. To characterize molecular mechanisms of CIRL functioning, we searched for its intracellular partners using the yeast two-hybrid SR system with the cytoplasmic C-terminal fragment of CIRL as bait. One of the interacting proteins was identified as TRIP8b, a putative cytosolic adapter protein with multiple tetratricopeptide repeats. To understand functional significance of CIRL-TRIP8b interaction, we further isolated TRIP8b-interacting proteins by affinity chromatography of brain extracts on immobilized recombinant TRIP8b. Sixteen proteins were identified by mass spectrometry in the purified preparations. Clathrin and subunits of AP2 complex appeared to be the major TRIP8b-interacting proteins. Our data suggest a role of TRIP8b in receptor-mediated endocytosis.

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Abbreviations

CIRL:

calcium-independent receptor of α-latrotoxin

GPCR:

G-protein coupled receptor

GPS:

GPCR proteolysis site

FERM:

RhoGEF and pleckstrin domain-containing protein 2

GST:

glutathione S-transferase

HCN channel:

hyperpolarization-activated cyclic nucleotide-regulated channel

IPTG:

isopropyl-β-D-thiogalactopyranoside

Ndufa9:

NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 9

NIPSNAP:

4-nitrophenylphosphatase domain and non-neuronal SNAP-25-like protein

PDZ:

domain name composed of first letters of names of three homologous proteins (post synaptic density protein, PSD-95; Drosophila disc large protein, Dlg; zonula occludens-1, ZO-1)

PMSF:

phenylmethylsulfonyl fluoride

PTS1:

peroxisome-targeting signal 1

Rab:

Ras-related proteins in brain

SOS:

Son of sevenless

SR system:

SOS recruitment system

TRIP8b:

TPR-containing Rab8b interacting protein

TPR:

tetratricopeptide repeat

References

  1. Krasnoperov, V. G., Bittner, M. A., Beavis, R., Kuang, Y., Salnikow, K. V., Chepurny, O. G., Little, A. R., Plotnikov, A. N., Wu, D., Holz, R. W., and Petrenko, A. G. (1997) Neuron, 18, 925–937.

    Article  PubMed  CAS  Google Scholar 

  2. Lelianova, V. G., Davletov, B. A., Sterling, A., Rahman, M. A., Grishin, E. V., Totty, N. F., and Ushkaryov, Y. A. (1997) J. Biol. Chem., 272, 21504–21508.

    Article  PubMed  CAS  Google Scholar 

  3. Krasnoperov, V., Lu, Y., Buryanovsky, L., Neubert, T. A., Ichtchenko, K., and Petrenko, A. G. (2002) J. Biol. Chem., 277, 46518–46526.

    Article  PubMed  CAS  Google Scholar 

  4. Hayflick, J. S. (2000) J. Recept. Signal Transduct. Res., 20, 119–131.

    Article  PubMed  CAS  Google Scholar 

  5. Davletov, B. A., Shamotienko, O. G., Lelianova, V. G., Grishin, E. V., and Ushkaryov, Y. A. (1996) J. Biol. Chem., 271, 23239–23245.

    Article  PubMed  CAS  Google Scholar 

  6. Krasnoperov, V. G., Beavis, R., Chepurny, O. G., Little, A. R., Plotnikov, A. N., and Petrenko, A. G. (1996) Biochem. Biophys. Res. Commun., 227, 868–875.

    Article  PubMed  CAS  Google Scholar 

  7. Rosenthal, L., and Meldolesi, J. (1989) Pharmacol. Ther., 42, 115–134.

    Article  PubMed  CAS  Google Scholar 

  8. Bittner, M. A., and Holz, R. W. (2000) J. Biol. Chem., 275, 25351–25357.

    Article  PubMed  CAS  Google Scholar 

  9. Bittner, M. A., Krasnoperov, V. G., Stuenkel, E. L., Petrenko, A. G., and Holz, R. W. (1998) J. Neurosci., 18, 2914–2922.

    PubMed  CAS  Google Scholar 

  10. Lang, J., Ushkaryov, Y., Grasso, A., and Wollheim, C. B. (1998) EMBO J., 17, 648–657.

    Article  PubMed  CAS  Google Scholar 

  11. Tobaben, S., Sudhof, T. C., and Stahl, B. (2000) J. Biol. Chem., 275, 36204–36210.

    Article  PubMed  CAS  Google Scholar 

  12. Kreienkamp, H. J., Soltau, M., Richter, D., and Bockers, T. (2002) Biochem. Soc. Trans., 30, 464–468.

    Article  PubMed  CAS  Google Scholar 

  13. Chen, S., Liang, M. C., Chia, J. N., Ngsee, J. K., and Ting, A. E. (2001) J. Biol. Chem., 276, 13209–13216.

    Article  PubMed  CAS  Google Scholar 

  14. Santoro, B., Wainger, B. J., and Siegelbaum, S. A. (2004) J. Neurosci., 24, 10750–10762.

    Article  PubMed  CAS  Google Scholar 

  15. Aronheim, A., Zandi, E., Hennemann, H., Elledge, S. J., and Karin, M. (1997) Mol. Cell. Biol., 17, 3094–3102.

    PubMed  CAS  Google Scholar 

  16. Colicelli, J., Birchmeier, C., Michaeli, T., O’Neill, K., Riggs, M., and Wigler, M. (1989) Proc. Natl. Acad. Sci. USA, 86, 3599–3603.

    Article  PubMed  CAS  Google Scholar 

  17. Brinkmann, U., Mattes, R. E., and Buckel, P. (1989) Gene, 85, 109–114.

    Article  PubMed  CAS  Google Scholar 

  18. Terry, D. E., Umstot, E., and Desiderio, D. M. (2004) J. Am. Soc. Mass Spectrom., 15, 784–794.

    Article  PubMed  CAS  Google Scholar 

  19. Pearse, B. M., and Robinson, M. S. (1984) EMBO J., 3, 1951–1957.

    PubMed  CAS  Google Scholar 

  20. D’Andrea, L. D., and Regan, L. (2003) Trends Biochem. Sci., 28, 655–662.

    Article  PubMed  CAS  Google Scholar 

  21. Amery, L., Sano, H., Mannaerts, G. P., Snider, J., van Looy, J., Fransen, M., and van Veldhoven, P. P. (2001) Biochem. J., 357, 635–646.

    Article  PubMed  CAS  Google Scholar 

  22. Subramani, S. (1998) Physiol. Rev., 78, 171–188.

    PubMed  CAS  Google Scholar 

  23. Lau, A. S., and Mruk, D. D. (2003) Endocrinology, 144, 1549–1563.

    Article  PubMed  CAS  Google Scholar 

  24. Brodin, L., Low, P., and Shupliakov, O. (2000) Curr. Opin. Neurobiol., 10, 312–320.

    Article  PubMed  CAS  Google Scholar 

  25. Reiter, E., and Lefkowitz, R. J. (2006) Trends Endocrinol. Metab., 17, 159–165.

    Article  PubMed  CAS  Google Scholar 

  26. Blondeau, F., Ritter, B., Allaire, P. D., Wasiak, S., Girard, M., Hussain, N. K., Angers, A., Legendre-Guillemin, V., Roy, L., Boismenu, D., Kearney, R. E., Bell, A. W., Bergeron, J. J., and McPherson, P. S. (2004) Proc. Natl. Acad. Sci. USA, 101, 3833–3838.

    Article  PubMed  CAS  Google Scholar 

  27. Xiong, Y., and Steitz, T. A. (2006) Curr. Opin. Struct. Biol., 16, 12–17.

    Article  PubMed  CAS  Google Scholar 

  28. Seroussi, E., Pan, H. Q., Kedra, D., Roe, B. A., and Dumanski, J. P. (1998) Gene, 212, 13–20.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Shemyakin—Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117991, Moscow, Russia

    N. V. Popova, R. Kh. Ziganshin, I. E. Deyev & A. G. Petrenko

  2. New York University, 10016, New York, USA

    A. N. Plotnikov & A. G. Petrenko

Authors
  1. N. V. Popova
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  2. A. N. Plotnikov
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  3. R. Kh. Ziganshin
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  4. I. E. Deyev
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  5. A. G. Petrenko
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Correspondence to N. V. Popova.

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Original Russian Text © N. V. Popova, A. N. Plotnikov, R. Kh. Ziganshin, I. E. Deyev, A. G. Petrenko, 2008, published in Biokhimiya, 2008, Vol. 73, No. 6, pp. 804–812.

Originally published in Biochemistry (Moscow) On-Line Papers in Press, as Manuscript BM07-367, April 13, 2008.

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Popova, N.V., Plotnikov, A.N., Ziganshin, R.K. et al. Analysis of proteins interacting with TRIP8b adapter. Biochemistry Moscow 73, 644–651 (2008). https://doi.org/10.1134/S0006297908060035

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  • DOI: https://doi.org/10.1134/S0006297908060035

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