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Genetically encoded intracellular sensors based on fluorescent proteins

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Abstract

Green fluorescent protein from Aequorea victoria and its many homologs are now widely used in basic and applied research. These genetically encoded fluorescent markers can detect localization of cell proteins and organelles in living cells and also cells and tissues in living organisms. Unique instruments and methods for studies of molecular biology of a cell and high throughput drug screenings are based on fluorescent proteins. This review deals with the most intensively evolving directions in this field, the development of genetically encoded sensors. Changes in their spectral properties are used for monitoring of cell enzyme activities or changes in concentrations of particular molecules.

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Abbreviations

BFP:

blue fluorescent protein

CaM:

calmodulin

CFP:

cyan fluorescent protein

EGFP:

enhanced green fluorescent protein

EGFR:

epidermal growth factor receptor

FLIM:

fluorescence lifetime imaging microscopy

FP:

fluorescent protein

FRET:

Forster resonance energy transfer

GES:

genetically encoded sensors

GFP:

green fluorescent protein

GKI:

protein kinase G

MLCK:

myosin light chain kinase

M13:

calmodulin-binding fragment of MLCK

PDE:

phosphodiesterase

PKA:

protein kinase A

TnC:

troponin C

YC:

Yellow Cameleons

YFP:

yellow fluorescent protein

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Authors and Affiliations

  1. Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117997, Moscow, Russia

    E. A. Souslova & D. M. Chudakov

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  1. E. A. Souslova
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  2. D. M. Chudakov
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Correspondence to D. M. Chudakov.

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Original Russian Text © E. A. Souslova, D. M. Chudolkov, 2007, published in Biokhimiya, 2007, Vol. 72, No. 7, pp. 837–855.

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Souslova, E.A., Chudakov, D.M. Genetically encoded intracellular sensors based on fluorescent proteins. Biochemistry Moscow 72, 683–697 (2007). https://doi.org/10.1134/S0006297907070012

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