Abstract
Conditions of conversion of 17α-methyltestosterone to methandrostenolone with the presence of modified β-cyclodextrins (methylcyclodextrin, hydroxypropylcyclodextrin, and hydroxyethylcyclodextrin) in the steroid: cyclodextrin ratio 1: 1 were studied. The experimental solutions of modified β-cyclodextrins were prepared in deionized water with 5–7% methanol. Under the conditions found to be optimal, 1,2–dehydrogenation of 17α-methyltestosterone was carried out with 2–4 g/l Pimelobacter simplex VKPM Ac-1632 biomass. At the substrate concentration 5–20 g/l, the reaction occurred for 1–15 h without any by-products. The maximum rate of methandrostenolone accumulation was observed with hydroxypropylcyclodextrin. The methylcyclodextrin solution can be reused for complete 17α-methyltestosterone conversion at the concentration 5 g/l.
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Original Russian Text © A.V. Druzhinina, V.A. Andryushina, T.S. Stytsenko, N.E. Voishvillo, 2008, published in Prikladnaya Biokhimiya i Mikrobiologiya, 2008, Vol. 44, No. 6, pp. 642–646.
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Druzhinina, A.V., Andryushina, V.A., Stytsenko, T.S. et al. Conversion of 17α-methyltestosterone to methandrostenolone by the bacterium pimelobacter simplex VKPM Ac-1632 with the presence of cyclodextrins. Appl Biochem Microbiol 44, 580–584 (2008). https://doi.org/10.1134/S0003683808060045
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DOI: https://doi.org/10.1134/S0003683808060045