Human Cell

, Volume 23, Issue 4, pp 156–163

Characterization of two independent, exposure-time dependent paclitaxel-resistant human ovarian carcinoma cell lines

Authors

  • Kuninobu Nakajima
    • Department of Obstetrics/GynecologyJikei University School of Medicine
    • Department of Obstetrics/GynecologyJikei University Daisan Hospital
  • Misato Saito
    • Department of Obstetrics/GynecologyJikei University School of Medicine
  • Toshiaki Tachibana
    • Department of AnatomyJikei University School of Medicine
  • Hiroshi Ishikawa
    • Laboratory of Regenerative Medical Sciences, School of Life Dentistry at TokyoThe Nippon Dental University
Cell Line

DOI: 10.1111/j.1749-0774.2010.00098.x

Cite this article as:
Nakajima, K., Isonishi, S., Saito, M. et al. Hum Cell (2010) 23: 156. doi:10.1111/j.1749-0774.2010.00098.x

Abstract

This experiment was conducted to address the question of whether acquired paclitaxel resistance is dependent upon whether it is given as a single brief exposure or as a long-term exposure. PX2 and PX24 were established from 2008 human ovarian cancer cells by 2-h single exposure or 24-h continuous exposure to paclitaxel. PX2 acquired paclitaxel resistance faster than PX24 by twofold. Drug resistant pattern was exposure-time dependent. In 2-h exposure, PX2 showed 53.86 ± 4.96 (mean ± standard deviation [SD]) fold paclitaxel resistance while PX24 showed 9.51 ± 1.01 fold resistance (P = 0.002). In 24-h exposure, PX2 showed 2.31 ± 0.3 fold paclitaxel resistance while PX24 showed 28.1 7 ± 0.98 fold resistance (P = 0.040). PX2 and PX24 acquired cross-resistance to docetaxel and SN38 and the resistance degrees were significantly higher in PX2 than PX24. They displayed approximately twofold cisplatin collateral sensitivity. PX24 also displayed sensitivity to other platinum drugs, oxaliplatin and ZD0473, whereas PX2 acquired significant resistance to both of them. Although differential tubulin-isotype expressions were noted among 2008, PX2 and PX24, they were not significant. In electron microscopy, prominent, densely stained lysosomes were observed more in the resistant cells than 2008. Two independent, exposure-time dependent paclitaxel-resistant human ovarian carcinoma cell lines were established. Understanding the characteristics of the differential resistance pattern could be clinically beneficial for the selection of second line chemotherapy for relapsed ovarian cancer.

Key words

beta-tubulinecytotoxic patternexposure-time dependentovarian carcinoma cellspaclitaxel-resistant

Copyright information

© Society and Springer Japan 2010