Human Cell

, Volume 23, Issue 1, pp 26–34

Heterotopic autotransplantation of ovarian cortex in cynomolgus monkeys

  • Suguru Igarashi
  • Nao Suzuki
  • Shu Hashimoto
  • Seido Takae
  • Makoto Takenoshita
  • Yoshihiko Hosoi
  • Yoshiharu Morimoto
  • Bunpei Ishizuka
Research Article

DOI: 10.1111/j.1749-0774.2010.00081.x

Cite this article as:
Igarashi, S., Suzuki, N., Hashimoto, S. et al. Hum Cell (2010) 23: 26. doi:10.1111/j.1749-0774.2010.00081.x
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Abstract

In recent years, removal of ova or ovaries before chemotherapy or radiation therapy has been investigated in young female cancer patients to avoid the adverse effects of treatment. Orthotopic autotransplantation of ovarian cortex has advantages such as easy collection of ova and the possibility of spontaneous pregnancy. Although children have been born after successful orthotopic autotransplantation into the residual ovaries, some patients cannot undergo this procedure such as those who need bilateral ovariectomy or pelvic radiation therapy, therefore it is still necessary to investigate suitable heterotopic autotransplantation sites. The present study was performed in primates (cynomolgus monkeys) with the objective of determining the optimum site for heterotopic autotransplantation of ovarian cortex to enhance the clinical application of this method. The retroperitoneal iliac fossa and omentum were selected as sites for heterotopic autotransplantation. Two cynomolgus monkeys were subjected to laparotomy under anesthesia. After resection of the bilateral adnexae, the ovaries were cut into 0.5 cm cubes that were transplanted. Blood levels of follicle-stimulating hormone, luteinizing hormone, estradiol, and progesterone were monitored, and monkeys with a regular estrus cycle underwent superovulation and egg collection. In both monkeys studied, recovery of a regular estrus cycle was confirmed after heterotopic autotransplantation of ovarian tissue. Mll phase ova were successfully collected from tissues transplanted into the retroperitoneal iliac fossa or omentum. Development to the early blastocyst stage was confirmed after microfertilization. We established an appropriate method of heterotopic autotransplantation using ovarian cortex into the retroperitoneal iliac fossa or omentum in primates.

Key words

cynomolgus monkeyfertility preservationheterotopic autotransplantationomentum

Copyright information

© Society and Springer Japan 2010

Authors and Affiliations

  • Suguru Igarashi
    • 1
  • Nao Suzuki
    • 1
  • Shu Hashimoto
    • 2
  • Seido Takae
    • 1
  • Makoto Takenoshita
    • 3
  • Yoshihiko Hosoi
    • 4
  • Yoshiharu Morimoto
    • 2
  • Bunpei Ishizuka
    • 1
  1. 1.Department of Obstetrics and GynecologySt. Marianna University School of MedicineKanagawaJapan
  2. 2.IVF Namba ClinicNishi-ku
  3. 3.EvebioscienceWakayamaJapan
  4. 4.Department of Genetic EngineeringKinki UniversityOsaka