Fisheries Science

, Volume 74, Issue 3, pp 677–686

The occurrence of tissue-specific twitchin isoforms in the mussel Mytilus galloprovincialis

  • Miho Kusaka
  • Daisuke Ikeda
  • Daisuke Funabara
  • David J Hartshorne
  • Shugo Watabe
Article

DOI: 10.1111/j.1444-2906.2008.01574.x

Cite this article as:
Kusaka, M., Ikeda, D., Funabara, D. et al. Fish Sci (2008) 74: 677. doi:10.1111/j.1444-2906.2008.01574.x

Abstract

The catch state in Mytilus anterior byssus retractor muscle is regulated by phosphorylation and dephosphorylation of twitchin, a member of the titin/connectin superfamily, and involves two serine residues, Ser-1075 (D1) and Ser-4316 (D2). This study was undertaken to examine whether isoforms of twitchin were expressed in various muscles of the mussel Mytilus galloprovincialis by reverse transcription-polymerase chain reaction. Mussel tissues, including both catch and non-catch muscles, contained various twitchin isoforms that all contained the D2 site and the kinase domain. However, sequence alterations were detected around the D1 site, notably a potential deletion of the D1 site. All isoforms from catch muscles contained both the D1 and D2 sites, whereas those from non-catch muscles also expressed the D2 site, but some of them lacked the D1 site. This suggests that the D1 site of twitchin is essential to the mechanism of catch. Genomic DNA analysis revealed that twitchin isoforms are produced by alternative splicing.

Key words

anterior byssus retractor musclecatchisoformmolluskmusselMytilus galloprovincialistwitchin

Copyright information

© The Japanese Society of Fisheries Science 2008

Authors and Affiliations

  • Miho Kusaka
    • 1
  • Daisuke Ikeda
    • 1
  • Daisuke Funabara
    • 2
  • David J Hartshorne
    • 3
  • Shugo Watabe
    • 1
  1. 1.Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life SciencesThe University of TokyoBunkyo, TokyoJapan
  2. 2.Laboratory of Muscle Biology Graduate School of BioresourcesMie UniversityTsu, MieJapan
  3. 3.Muscle Biology GroupUniversity of ArizonaTucsonUSA