Journal of NeuroVirology

, Volume 13, Issue 4, pp 338–346

Characterization of JC virus in cerebrospinal fluid from HIV-1 infected patients with progressive multifocal leukoencephalopathy: insights into viral pathogenesis and disease prognosis

  • Angela Marzocchetti
  • Maurizio Sanguinetti
  • Simona Di Giambenedetto
  • Antonella Cingolani
  • Giovanni Fadda
  • Roberto Cauda
  • Andrea De Luca
Article

DOI: 10.1080/13550280701381324

Cite this article as:
Marzocchetti, A., Sanguinetti, M., Di Giambenedetto, S. et al. Journal of NeuroVirology (2007) 13: 338. doi:10.1080/13550280701381324

Abstract

Objectives. To analyze virological and immunological features of AIDS-related progressive multifocal leukoencepalophathy (PML) and their association to disease prognosis. Methods. In HIV-infected patients with virologically confirmed PML, JC virus (JCV) DNA load and levels of Macrophage Chemoattractant Protein (MCP)-1 were determined in cerebrospinal fluid. JCV genotypes, rearrangements and JCV DNA binding sites for cellular transcription factors were analyzed by sequencing the viral VP1 region and regulatory region (RR). Results. 45 patients were analyzed: 60% were exposed to highly active antiretroviral therapy (HAART) after PML and 24% before the disease onset. JCV DNA load in cerebrospinal fluid was a strong predictor of patients survival. Lower levels of JCV DNA in cerebrospinal fluid were associated with the following virologic factors: viral genotype 4 (p = 0.043), more rearrangements in the RR (p = 0.046), duplication of RR block B (p = 0.028), and duplication of binding sites for cellular transcription factor NF-1 (p = 0.060). In patients with prior antiretroviral exposure there was a trend towards a higher number of binding sites for cellular transcription factors (p = 0.068). Lower JCV load was also predicted by exposure to HAART (p = 0.010), higher baseline CD4 counts (p = 0.009) and higher cerebrospinal fluid MCP-1 levels (p = 0.036). In a multiple regression model, MCP-1 levels were independently associated with JCV load. Conclusion. HAART leads to a partial immune-mediated control of JCV replication; the virus may tend to escape through the selection of rearrangements in the RR, some associated with enhanced viral replication efficiency, other resulting in multiplication of binding sites for cellular transcription factors.

Keywords

JC Virus progressive multifocal leukoencephalopathy cerebrospinal fluid 

Copyright information

© Journal of NeuroVirology, Inc. 2007

Authors and Affiliations

  • Angela Marzocchetti
    • 1
    • 3
  • Maurizio Sanguinetti
    • 2
  • Simona Di Giambenedetto
    • 1
  • Antonella Cingolani
    • 1
  • Giovanni Fadda
    • 2
  • Roberto Cauda
    • 1
  • Andrea De Luca
    • 1
  1. 1.Institute of Clinical Infectious DiseasesCatholic University of Sacred HeartRomeItaly
  2. 2.Institute of MicrobiologyCatholic University of Sacred HeartRomeItaly
  3. 3.Division of Viral Pathogenesis, Beth Israel Deaconess CenterHarvard Medical SchoolBoston

Personalised recommendations