Journal of NeuroVirology

, Volume 8, Issue 4, pp 345–352

The rabies virus glycoprotein determines the distribution of different rabies virus strains in the brain


  • Xiuzhen Yan
    • Department of Pathology, College of Veterinary MedicineThe University of Georgia
  • Puliyur S. Mohankumar
    • Neuroendocrine Research Laboratory, Department of Pathobiology and Diagnostic InvestigationMichigan State University
  • Bernhard Dietzschold
    • Department of Microbiology and ImmunologyThomas Jefferson University
  • Matthies J. Schnell
    • Department of Biochemistry and Molecular PharmacologyThomas Jefferson University
    • Department of Pathology, College of Veterinary MedicineThe University of Georgia
Short Communication

DOI: 10.1080/13550280290100707

Cite this article as:
Yan, X., Mohankumar, P.S., Dietzschold, B. et al. Journal of NeuroVirology (2002) 8: 345. doi:10.1080/13550280290100707


The contribution of rabies virus (RV) glycoprotein (G) in viral distribution in the brain was examined by immunohistochemistry following stereotaxic inoculation into the rat hippocampus. Viruses used in this study include the highly neuroinvasive challenge virus standard strains (CVS-N2C and CVS-B2C) and the nonneuroinvasive attenuated SN-10 strain, as well as SN-10-derived recombinant viruses expressing the G gene from CVS-N2C (RN2C) or CVS-B2C (RB2C). The distribution of recombinant viruses in the brain was similar to those of the parental viruses from which the G was derived. For example, while CVS-B2C- and RB2C-infected neurons were seen preferentially in the hippocampus, cortex, and hypothalamus, CVS-N2C- and RN2C-infected neurons were preferentially found in the hippocampus, cortex, and thalamus. SN-10 infected efficiently almost all the brain regions. To further study the role of the RV G in virus spreading, we examined the distribution of RV antigen in brains infected with a recombinant RV in which the SN-10 G was replaced with vesicular stomatitis virus (VSV) G (SN-10-VG) was examined. The spreading of SN-10-VG to the cortex and the thalamus was drastically reduced, but the number of infected neurons in hippocampus and hypothalamus, particularly the paraventricular nucleus, was similar to the SN-10 virus. This pattern of spreading resembles that of VSV. Together, our data demonstrate that it is the G protein that determines the distribution pattern of RV in the brain.


rabies virusspreadingdistributionglycoproteinreverse genetics

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© Taylor & Francis 2002