CD4/CXCR4-independent infection of human astrocytes by a T-tropic strain of HIV-1
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
HIV-1 establishes a low-level persistent infection in astrocytes. In this study, we studied the susceptibility of a human astrocyte cell line (SVG-A) to infection with luciferase expressing reporter viruses pseudotyped with envelopes derived from five isolates of HIV-1. SVG-A cells were susceptible to infection by a T-cell tropic isolate and the infection was both CD4 and CXCR4 independent. These data confirm the susceptibility of astrocytes to infection with T-tropic strains of HIV-1 and suggest a novel mechanism by which T-tropic strains of HIV can infect cells.
- Albright AV, Shieh JTC, Itoh T, Lee B, Pleasure D, O’Connor MJ, Doms RW, Gonzalez-Scarano F (1999). Microglia express CCR5, CXCR4, and CCR3, but, of these, CCR5 is the principal coreceptor for HIV-1 dementia isolates. J Virol 73: 205–213.
- Alkhatib F, Combadiere C, Broder C, Feng Y, Kennedy PE, Murphy PM, Berger EA (1996). CC CKR5: a RANTES, MIP-1 alpha, MIP-1 beta receptor as a fusion cofactor for macrophage-tropic HIV-1. Science 272: 1955–1958. CrossRef
- An SF, Groves M, Giometto B, Beckett AAJ, Scaravelli F (1999a). Detection and localisation of HIV-1 DNA and RNA in fixed adult AIDS brain by polymerase chain reaction/insitu hybridization technique. Acta Neuropathol 98: 481–487. CrossRef
- An SF, Groves M, Gray F, Scaravilli F (1999b). Early entry and widespread cellular involvement of HIV-1 DNA in brains of HIV-1 positive asymptomatic individuals. J Neuropathol Exp Neurol 58(11): 1156–1162. CrossRef
- Atwood WJ, Berger JR, Kaderman R, Tornatore CS, Major EO (1993). Human immunodeficiency virus type 1 infection of the brain. Clin Microbiol Rev 6: 339–366.
- Bagasra O, Lavi E, Bobroski L, Khalili K, Pestaner JP, Pomerantz RJ (1996). Cellular reservoirs of HIV-1 in the central nervous system of infected individuals: identification by the combination of in situ PCR and immunohistochemistry. AIDS 10: 573–585. CrossRef
- Bencheikh M, Bentsman G, Sarkissian N, Canki M, Volsky DJ (1999). Replication of different clones of human immunodeficiency virus type 1 in primary fetal human astrocytes: enhancement of viral gene expression by Nef. J NeuroVirol 5: 115–124. CrossRef
- Brack-Werner R (1999). Astrocytes: HIV cellular reservoirs and important participants in neuropathogenesis. AIDS 13: 1–22. CrossRef
- Conant K, Tornatore CS, Atwood WJ, Meyers K, Traub R, Major EO (1994). In vivo and in vitro infection of the astrocyte by HIV-1. Adv Neuroimmunol 4: 1–3. CrossRef
- Deng H, Liu R, Ellmeier W, Choe S, Unatmaz D, Burkhart M, DiMarzio P, Marmon S, Sutton RE, Hill CM, Davis CB, Peiper SC, Schall TJ, Littman DR, Landau NR (1996). Identification of a major co-receptor for primary isolates of HIV-1. Nature 381: 661–666. CrossRef
- DiRienzo AM, Aloisi F, Santarcangelo AC, Palladino C, Olivetta E, Genovese D, Verani P, Levi G (1998). Virological and molecular parameters of HIV-1 infection of human embryonic astrocytes. Arch Virol 143: 1599–1615. CrossRef
- Doranz BJ, Rucker J, Yi Y, Smith RJ, Samson M, Peiper SC, Parmentier M, Collman RG, Doms RW (1996). A dual tropic primary HIV-1 isolate that used fusin and the beta-chemokine receptors CCR5, CCR3 and CKR-2b as fusion cofactors. Cell 85: 1149–1158. CrossRef
- Epstein L, Gendelman H (1993). Human immunodeficiency virus type 1 infection of the nervous system: pathogenetic mechanisms. Ann Neurol 33: 429–436. CrossRef
- Everall I, Luthert P, Lantos P (1991). Neuronal loss in the frontal cortex in HIV infection. Lancet 337: 1119–1121. CrossRef
- Gabuzda D, Ho D, de la Monte S, Hirsch M, Rota T, Sobel R (1986). Immunohistochemical identification of HTLV-III antigen in brains of patients with AIDS. Ann Neurol 20: 289–295. CrossRef
- Hao H, Lyman WD (1999). HIV infection of fetal human astrocytes: the potential role of a receptor-mediated endocytic pathway. Brain Res 823: 24–32. CrossRef
- He J, Chen Y, Farzan M, Choe H, Ohagen A, Gartner S, Busciglio J, Yang X, Hofmann W, Newman W, Mackay CR, Sodroski J, Gabuzda D (1997). CCR3 and CCR5 are co-receptors for HIV-1 infection of microglia. Nature 385: 645–649. CrossRef
- Jordan CA, Watkins BA, Kufta C, Dubois-Dalcq M (1991). Infection of brain microglial cells by human immuno-deficiency virus type 1. J Virol 65: 736–742.
- Keir SD, Miller J, Yu G, Hamilton R, Samulski RJ, Xiao X, Tornatore C (1997). Efficient gene transfer into primary and immortalized human fetal glial cells using adenoassociated virus vectors: establishment of a glial cell line with a functional CD4 receptor. J Neurovirol 3: 322–330. CrossRef
- Koenig S, Gendelman H, Orenstein J, Dal Canto M, Pezeshkpour G, Yungbluth M, Janotta F, Aksamit A, Martin M, Fauci A (1986). Detection of AIDS virus in macrophages in brain tissue from AIDS patients. Science 233: 1089–1093. CrossRef
- Kolson DL, Lavi E, Gonzalez-Scarano F (1998). The effects of human immunodeficiency virus in the central nervous system. Adv Virus Res 50: 1–700. CrossRef
- Lipton SA (1994). HIV-related neuronal injury. Mol Neurobiol 8: 181–196. CrossRef
- Ludwig E, Ceccherini-Silberstein F, van Empel J, Erfle V, Neumann M, Brack-Werner R (1999). Diminished rev-mediated stimulation of human immunodeficiency virus type 1 protein synthesis is a hallmark of human astrocytes. J Virol 73: 8279–8289.
- McArthur JC, McClernon DR, Cronin MF, Nance-Sproson TE, Saah AJ, St Clair M, Lanier ER (1997). Relationship between human immunodeficiency virus-associated dementia and viral load in cerebrospinal fluid and brain. Ann Neurol 42: 689–698. CrossRef
- Nath A, Hartloper V, Furer M, Fowke KR (1995). Infection of human fetal astrocytes with HIV-1: viral tropism and the role of cell to cell contact in viral transmission. J Neuropathol Exp Neurol 54: 320–330. CrossRef
- Nuovo GJ, Gallery F, MacConnell P, Braun A (1994). In situ detection of polymerase chain reaction-amplified HIV-1 nucleic acids and tumor necrosis factor-alpha RNA in the central nervous system. Am J Pathol 144: 659–666.
- Ranki A, Nyberg M, Ovod V (1995). Abundant expression of HIV Nef and Rev proteins in brain astrocytes in vivo is associated with dementia. AIDS 9: 1001–1008. CrossRef
- Sabri F, Tresoldi E, Di Stefano M, Polo S, Monaco MC, Verani A, Fiore JR, Lusso P, Major E, Chiodi F, Scarlatti G (1999). Nonproductive human immunodeficiency virus type 1 infection of human fetal astrocytes: independence from CD4 and major chemokine receptors. Virology 264: 370–384. CrossRef
- Shieh JTC, Albright AV, Sharron M, Gartner S, Strizki J, Doms RW, Gonzalez-Scarano F (1998). Chemokine receptor utilization by human immunodeficiency virus type 1 isolates that replicate in microglia. J Virol 72: 4243–4249.
- Tornatore C, Chandra R, Berger JR, Major EO (1994a). HIV-1 infection of subcortical astrocytes in the pediatric central nervous. Neurology 44: 481–487.
- Tornatore C, Meyers K, Atwood W, Conant K, Major E (1994b). Temporal patterns of human immunodeficiency virus type 1 transcripts in human fetal astrocytes. J Virol 68: 93–102.
- Tornatore C, Nath A, Amemiya K, Major EO (1993). Persistent human immunodeficiency virus type 1 infection in human fetal glial cells reactivated by T-cell factor(s) or by the cytokines tumor necrosis factor alpha and interleukin-1 beta. J Virol 65: 6094–6100.
- Tornatore CS, Nath A, Amemiya K, Major EO (1991). Persistent human immunodeficiency virus type 1 infection in human glial cells reactivated by T cell factors or by the cytokines tumor necrosis factor alpha and interleukin 1 beta. J Virol 65: 6094–6100.
- Watkins BA, Dorn HH, Kelly WB, Armstrong RC, Potts BJ, Michaels F, Kufta CV, Dubois-Dalcq M (1990). Specific tropism of HIV-1 for microglial cells in primary human brain cultures. Science 249: 549–552. CrossRef
- Wiley C, Schrier R, Nelson J, Lampert P, Oldstone M (1986). Cellular localization of human immunodeficiency virus infection within the brains of aquired immune deficiency patients. Proc Natl Acad Sci USA 83: 7089–7093. CrossRef
- CD4/CXCR4-independent infection of human astrocytes by a T-tropic strain of HIV-1
Journal of NeuroVirology
Volume 7, Issue 2 , pp 155-162
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Industry Sectors
- Author Affiliations
- 1. Department of Molecular Microbiology and Immunology, Brown University, 69 Brown Street, Box G-B616, 02192, Providence, RI, USA
- 2. Department of Neurology, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania, USA