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In vitro propagation of Albizia odoratissima L.F. (Benth.) from cotyledonary node and leaf nodal explants

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Summary

Cotyledonary node and leaf nodal explants excised from 14-d-old in vitro-grown seedlings of Albizia odoratissima were cultured on a Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP), N 6-(2-isopentenyl) adenine (2-iP) and kinetin, used either solely or in combinations. The highest frequency for shoot regeneration (82.5%), the maximum number of shoots per explant (6.9), and the maximum shoot length (2.55 cm) were obtained from cotyledonary node explants cultured on a MS medium containing 10 μM BAP and 10 μM 2-iP with 30 gl−1 sucrose. Successful rooting was achieved by placing the microshoots on MS medium with 25 μM indole-3-butyric acid (IBA) for 24h first, then transferring to the same medium without IBA. Of the various substrates tested, vermiculite was best for plant acclimatization, in which 75% of plants survived.

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Authors and Affiliations

  1. Department of Plant Sciences, School of Biological Sciences, Madurai Kamaraj University, 625 021, Madurai, Tamil Nadu, India

    V. Rajeswari & Kailash Paliwal

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  1. V. Rajeswari
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  2. Kailash Paliwal
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Correspondence to Kailash Paliwal.

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Rajeswari, V., Paliwal, K. In vitro propagation of Albizia odoratissima L.F. (Benth.) from cotyledonary node and leaf nodal explants. In Vitro Cell.Dev.Biol.-Plant 42, 399–404 (2006). https://doi.org/10.1079/IVP2006799

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  • DOI: https://doi.org/10.1079/IVP2006799

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