Influence of some adjuvants on in vitro clonal propagation of male and female jojoba plants
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
The influence of different adjuvants, activated charcoal (AC), casein hydrolyzate (CH), coconut water (CW), polyvinylpyrrolidone (PVP), and triiodobenzoic acid (TIBA), has been assessed on the shoot production potential of the nodal explants derived from in vitro-raised male and female jojoba (Simmondsia chinensis) shoots. Nodal explants of each sex were cultured separately on Murashige and Skoog medium supplemented with different levels of AC, CW, CH, PVP, and TIBA either alone or along with optimum levels of N 6-benzyladenine (BA; 10 μM for male, 20 μM for female). Some differences in response of the explants of both the sexes have been observed in terms of (1) percentage of explants developing shoots, (2) average shoot number, and (3) average shoot length. AC alone proved beneficial for elevating morphogenic response in male as well as female explants in comparison to basal medium or media containing AC and the optimum level of BA. When used alone, CH proved inhibitory for shoot differentiation in both sexes, especially in male explants. Addition of PVP to MS enhanced shoot proliferation in female explants only, but along with BA it increased the response of male explants. BA in combination with different levels of TIBA promoted shoot multiplication in female explants. Thus, explants of both male and female shoots exhibited differential morphogenic behavior under the influence of various adjuvants. However, BA alone proved to be the best for differentiation of shoots in both male (10 μM) as well as female (20 μM) explants.
- Agrawal, V.; Prakash, S.; Gupta, S. C.. Differential hormonal requirements for clonal propagation of male and female jojoba plants. In: Altman A.; Ziv, M.; Izhar, S., eds. Current science and biotechnology in agriculture: plant biotechnology and in vitro biology in the 21st century Dordrecht: Kluwer Academic Publishers; 1999:23–26.
- Anonymous, Jojoba: new crop for arid lands new raw material for industry. Washington, DC: National Academic Press; 1985.
- Benzioni, A. Flower bud dormancy: ABA concentration, and survival during frost of jojoba genotypes under water stress. J. Am. Soc. Hort. Sci. 117:976–980; 1992.
- Chaturvedi, H. C.; Sharma, M. In vitro production of cloned plants of jojoba Simmondsia chinensis (Link) Schneider through shoot proliferation in long term culture. Plant Sci. 63:199–207; 1989. CrossRef
- Dauphin-Guerin, B.; Teller, G.; Durand B. Different endogenous cytokinins between male and female Mercurialis annua L. Planta 148:124–129; 1980. CrossRef
- De Winnaar, W. Clonal propagation of papaya in vitro. Plant Cell Tiss. Organ Cult. 12:305–310; 1988. CrossRef
- Gentry, H. S. The natural history of jojoba, Simmondsia chinensis and its cultural aspects. Econ. Bot. 12:261–295; 1958.
- Gui, Y. L. Induction of callus and regeneration of plantlets in stem segments culture of Chinese gooseberry. Acta Bot. Sin. 21:339–344; 1979.
- Harsh, L. N.; Tewari, J. C.; Patwal, D. S.; Meena, G. L. Package and practices for cultivation of jojoba, Simmondsia chinensis in arid zone. Jodhpur, India: Central Arid Zone Research Institute; 1987.
- Hirsch, A. M.; Fortune, D.; Chalak, L.; Legave, J. M.; Chat, J.; Monet, R., Peroxidase test: a test for sex sereening in kiwifruit seedlings. Acta Hort. 444:89–95; 1997.
- Jacoboni, A.; Standardi, A. Tissue culture of jojoba Simmondsia chinensis, Link. Acta Hort. 212:557–560; 1987.
- Jordan, M. Micropropagation of papaya, (Carica spp.). In: Bajaj, Y. P. S., ed. Biotechnology in agriculture and forestry, vol. 18: high-tech and micropropagation II. Berlin: Springer-Verlag: 1992:441–459.
- Kohorn, L. U. Shoot morphology and reproduction in jojoba: advantages of sexual dimorphism. Ecology 75:2384–2394; 1994. CrossRef
- Litz, R. E.; Conover, R. A. Effect of sex type season and other factors on in vitro establishment and culture of Carica papaya L. explants. J. Am. Soc. Hort. Sci. 106:792–794; 1981.
- Llorente, D. E.; Apostolo, N. M. Effect of different growth regulators and genotypes on in vitro propagation of jojoba. NZ J. Crop Hort. Sci. 26:55–62; 1998.
- Mehra, P. N.; Cheema, G. S. Differential response of male and female Himalayan poplar Populus ciliata and P. alba in vitro. Phytomorphology 35:151–154; 1985.
- Mills, D.; Wenkart, S.; Benzioni, A. Micropropagation of Simmondsia chinensis (jojoba). In: Bajaj, Y. P. S., ed. Biotechnology in agriculture and forestry, vol. 40: high-tech and micropropagation. Berlin: Springer-Verlag; 1997:370–393.
- Mirov, N. T. Simmondsia or jojoba—a problem in economic botany. Econ. Bot. 6:41–47; 1952.
- Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497; 1962. CrossRef
- Rost, T. L.; Hinchee, M. A. W. Preliminary report of the production of callus, organogenesis and regeneration of jojoba Simmondsia chinensis, Link, Schneid, in tissue culture. J. Hort. Sci. 55:299–305; 1980.
- Influence of some adjuvants on in vitro clonal propagation of male and female jojoba plants
In Vitro Cellular & Developmental Biology - Plant
Volume 39, Issue 2 , pp 217-222
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Simmondsia chinensis
- morphogenic response
- Industry Sectors