Skip to main content
Log in

Multiple shoot formation and plant regeneration from stem nodal explants of Paphiopedilum orchids

  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Summary

Stem nodal explants of Paphiopedilum philippinense hybrids (hybrid PH59 and PH60) directly formed shoots when cultured on a modified half-strength Murashige and Skoog (1962) basal medium supplemented with a combination of 2,4-dichlorophenoxyacetic acid (2,4-D: 4.52 and 45.25 μM) and 1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea (TDZ; 0.45 and 4.54 μM) for 6 mo. On hormone-free basal medium, the percentages of explants with shoots were 33.3% and 0% and the shoot numbers per explant were 1 and 0 in hybrid PH59 and hybrid PH60, respectively. In hybrid PH59, 4.52 μM 2,4-D plus 0.45μM TDZ induced a higher percentage of explants with shoots and shoot number per explant than did the hormone-free treatment. In hybrid PH60, although 4.52 μM 2,4-D and 0.45 μM TDZ promoted shoot formation, the highest shoot number was found with 4.52 μM 2,4-D alone. Plantlets, each having several roots, were obtained from regenerated shoots after transferring onto hormone-free basal medium for 3 mo. The plantlets were potted in sphagnum moss, and acclimatized well in a greenhouse.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Arditfi, J.; Ernst, R. Micropropagation of orchids. New York: John Wiley & Son; 1993:682 pp.

    Google Scholar 

  • Chang, C.; Chang, W. C. Plant regeneration from callus culture of Cymbidium ensifolium var. misericors. Plant Cell Rep. 17:251–255; 1998.

    Article  CAS  Google Scholar 

  • Chen, J. T.; Chang, C.; Chang, W. C. Direct somatic embryogenesis from leaf explants of Oncidium ‘Gower Ramsey’ and subsequent plant regeneration. Plant Cell Rep. 19:143–149; 1999.

    Article  CAS  Google Scholar 

  • Chen, J. T.; Chang, W. C. Plant regeneration via embryo and shoot bud formation from flower-stalk explants of Oncidium ‘Sweet Sugar’. Plant Cell Tiss. Organ Cult. 62:95–100; 2000a.

    Article  CAS  Google Scholar 

  • Chen, J. T.; Chang, W. C. Efficient plant regeneration through somatic embryogenesis from callus cultures of Oncidium (Orchidaccae). Plant Sci. 160:87–93; 2000b.

    Article  PubMed  CAS  Google Scholar 

  • Chen, T. Y.; Chen, J. T.; Chang, W. C. Plant regeneration through direct shoot bud formation from leaf cultures of Paphiopedilum orchids. Plant Cell Tiss. Organ Cult. (in press); 2002.

  • Chen, Y.; Piluck, C. Effects of thidiazuron and N6-benzylaminopurine on shoot regeneration of Phalaenopsis. Plant Growth Regul. 16:99–101; 1995.

    Article  CAS  Google Scholar 

  • Chen, Y. C.; Chang, C.; Chang, W. C. A reliable protocol for plant regeneration from callus culture of Phalaenopsis. In Vitro Cell. Dev. Biol. Plant 36:420–423; 2000.

    Article  CAS  Google Scholar 

  • Duncan, D. B. Multiple range and multiple F test. Biometrics 11:1–42; 1955.

    Article  Google Scholar 

  • Ernst, R. Effects of thidiazuron on in vitro propagation of Phalaenopsis and Doritaenopsis (Orchidaceae). Plant Cell Tiss. Organ Cult. 39:273–275; 1994.

    Article  CAS  Google Scholar 

  • Huang, L. C. A procedure for asexual multiplication of Paphiopedilum in vitro. Am. Orchid Soc. Bull. 57:274–278; 1988.

    Google Scholar 

  • Huang, L. C.; Lin, C. J.; Kuo, C. I.; Huang, B. L.; Murashige, T. Paphiopedilum cloning in vitro. Sci. Hortic. 91:111–121; 2001.

    Article  Google Scholar 

  • Lin, Y. H.; Chang, C.; Chang, W. C. Plant regeneration from callus culture of a Paphiopedilum hybrid. Plant Cell Tiss. Organ Cult. 62:21–25; 2000.

    Article  CAS  Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:495–497; 1962.

    Article  Google Scholar 

  • Nayak, N. R.; Rath, S. P.; Patnaik, S. In vitro propagation of three epiphytic orchids, Cymbidium aloifolium (L.) Sw., Dendrobium aphyllum (Roxb.) Fisch. and Dendrobium moschatum (Buch-Ham) Sw. through thidiazuron-induced high frequency shoot proliferation. Sci. Hortic. 71:243–250; 1997.

    Article  Google Scholar 

  • Teob, E. S. Orchids of Asia. Singapore: Times Books International; 1989; 317 pp.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Wei-Chin Chang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Chen, TY., Chen, JT. & Chang, WC. Multiple shoot formation and plant regeneration from stem nodal explants of Paphiopedilum orchids. In Vitro Cell.Dev.Biol.-Plant 38, 595–597 (2002). https://doi.org/10.1079/IVP2002332

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1079/IVP2002332

Key words

Navigation