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Somatic embryogenesis and plantlet regeneration in mango (Mangifera indica L.)

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Summary

The ‘Carabao’ or ‘Manila Super’ mango (Mangifera indica L.), a virtually neglected fruit before the advent of KNO3 flower induction in the early 1970s, is now the third leading Philippine export fruit after banana and pineapple. To apply biotechnology for improvement, a reliable embryogenesis and regeneration protocol is required. We have developed a protocol for somatic embryogenesis and plantlet regeneration in mango: eight strains of ‘Carabao’ and two unidentified varieties, PHL 12384 and PHL 12378. Over 40 batches of nucellar explants from immature fruis (0.75–5.0 cm long) were cultured in vitro from April 1999 to April 2000. Two media were used, MMSE. Mango Medium for Somatic Embryo Induction, Proliferation and Germination and MMPR, Mango Medium for Plantlet Regeneration. These are now routinely used. The protocol is reproducible in 14 other varieties of mango. Shifting the base medium from Gamborg's B5 medium to our own formulation. BP medium (Barba and Pateña's formulation) effectively controlled browning. Browning has limited the successful in vitro culture of many woody species including the mango.

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  • Pateña, L. F.; Refuerzo, L. C.; Barba, R. C. A simple and reliable protocol for somatic embryogenesis and plantlet regeneration in mango (Mangifera indica L.). Oral paper presented at the Asian Agriculture Congress, Westin Philippine Plaza, Manila Philippines, April 24–27, Abstracts; 2001:94.

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Authors and Affiliations

  1. Institute of Plant Breeding, College of Agriculture, University of the Philippines Los Baños, College, 4031, Laguna, Philippines

    Lilian F. Pateña, Luzminda R. Carlos-Refuerzo & Ramon C. Barba

Authors
  1. Lilian F. Pateña
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  2. Luzminda R. Carlos-Refuerzo
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  3. Ramon C. Barba
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Corresponding author

Correspondence to Lilian F. Pateña.

Additional information

Crop Science Society of the Philippines (CSSP) 2001 Best Paper Award, Asian Agriculture Congress, Westin Philippine Plaza, Manila, Philippines, April 24–27, 2001 and Philippine Fruit Association 2000 Best Poster Award, 8th National Symposium. PCARRD, Los Baños, Laguna, Philippines, November 14–16, 2000.

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Pateña, L.F., Carlos-Refuerzo, L.R. & Barba, R.C. Somatic embryogenesis and plantlet regeneration in mango (Mangifera indica L.). In Vitro Cell.Dev.Biol.-Plant 38, 173–177 (2002). https://doi.org/10.1079/IVP2001275

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  • DOI: https://doi.org/10.1079/IVP2001275

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