Article

In Vitro Cellular & Developmental Biology - Plant

, Volume 42, Issue 6, pp 614-616

Effects of low nitrogen medium on endogenous changes in ethylene, auxins, and cytokinins in in vitro shoot formation from rhizomes of Cymbidium kanran

  • Yuki Ogura-TsujitaAffiliated withDivision of Agricultural Botany, Laboratory of Horticultural Science, Department of Plant Resources, Faculty of Agriculture, Kyushu University Email author 
  • , Hiroshi OkuboAffiliated withDivision of Agricultural Botany, Laboratory of Horticultural Science, Department of Plant Resources, Faculty of Agriculture, Kyushu University

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Summary

Shoot formation from rhizome explants of Cymbidium kanran was promoted on Murashige and Skoog (MS) medium: (1) with 1 mgl−1 (4.4μM) 6-benzyladenine (BA) and 0.1 mgl−1 (0.54μM) α-naphthaleneacetic acid (NAA); (2) with ethylene inhibitor (silver nitrate, AgNO3); or (3) by reducing ammonium nitrate (NH4NO3) and potassium nitrate (KNO3) to 25 and 50%, respectively, of their original concentrations. Shoot formation by BA and NAA was strongly inhibited with the application of ethephon, an ethylene releaser. The ethylene production from the rhizome explants was reduced 30–55% on low nitrogen medium after 1–3 mo. of culture and 52% on BA and NAA medium after 1 mo. of culture compared with explants on standard MS medium. No difference in endogenous auxin (indole-3-acetic acid, IAA) and cytokinin (isopentenyl adenosine, iPA) contents in the rhizomes was found between the treatments. Low ethylene levels were correlated with higher frequency of shoot formation from the rhizomes.

Key words

ammonium nitrate indole-3-acetic acid isopentenyl adenosine potassium nitrate