In Vitro Cellular & Developmental Biology - Plant

, Volume 42, Issue 6, pp 558–561

In vitro propagation of endangered Lippia filifolia mart. and schauer ex schauer


    • Departamento de BotânicaICB, UFJF
  • Fátima Regina Gonçalves Salimena
    • Departamento de BotânicaICB, UFJF
  • Marcelo de Oliveira Santos
    • Departamento de BiologiaICB, UFJF
  • Leonardo da Silva Garcia
    • Departamento de BotânicaICB, UFJF
  • Patrícia Maria de Oliveira Pierre
    • Departamento de BiologiaICB, UFJF
  • Lyderson Facio Viccini
    • Departamento de BiologiaICB, UFJF
  • Wagner Campos Otoni
    • Departamento de Biologia VegetalCCB, UFV

DOI: 10.1079/IVP2006814

Cite this article as:
Peixoto, P.H.P., Salimena, F.R.G., Santos, M.d.O. et al. In Vitro Cell.Dev.Biol.-Plant (2006) 42: 558. doi:10.1079/IVP2006814


This work describes an efficient micropropagation protocol of Lippia filifolia. Nodal segments cultivation in MS medium supplemented with 6-benzylaminopurine (4.5 μM)/α-naphthaleneacetic acid (NAA; 54nM) induced multiple shoots (in average 27 shoots per explant). Elongated shoots were rooted with NAA (0.11 μM) and they maintained ploidy level of the in vitro produced explants. The basic chromosome number were 2n=2x=24. Regenerated rooted shoots were successfully acclimatized under shading house conditions. This is the first report involving the establishment of a protocol for shoot multiplication and rooting for endangered L. filifolia, contributing for germplasm preservation of this species.

Key words

Chromosome stabilityconservationmedicinal plantmicropropagationVerbenaceae

Copyright information

© Society for In Vitro Biology 2006