Article

In Vitro Cellular & Developmental Biology - Plant

, Volume 42, Issue 6, pp 534-537

First online:

Calluses of Cynara cardunculus Var. Cardunculus cardoon (Asteraceae): determination of cynarine and chlorogenic acid by automated high-performance capillary electrophoresis

  • Silvia P. TrajtembergAffiliated withDepartmento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria
  • , Nancy M. ApóstoloAffiliated withDepartmento de Ciencias Básicas, Universidad Nacional de Luján Email author 
  • , Graciela FernándezAffiliated withDepartmento de Ciencias Básicas, Universidad Nacional de Luján

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Summary

Cynara cardunculus var. cardunculus L., also known as cardoon, is a perennial weed naturalized in the Pampas region of Argentina. A quantification of cynarine and chlorogenic acid of callus and leaves from cardoon was performed by means of micellar electrokinetic capillary chromatography, showing that the content of cynarine is higher in calluses than in vivo leaves. The scavenging effect of the callus extract, determined by the thiobarbituric acid method, demonstrated its significant antioxidant capacity. The obtained results revealed that in vitro tissue culture is an excellent tool for producing cynarine for therapeutical purposes.

Key words

antixidant activity dicaffeoylquinic acids micellar electrokinetic capillary chromatography (MEKC) monodicaffeoylquinic acids thistle