Plant regeneration of Chorispora bungeana via somatic embryogenesis

  • Jianhui Wang
  • Lizhe An
  • Ruoyu Wang
  • Daqun Yang
  • Jing Si
  • Xuanying Fu
  • Jianfeng Chang
  • Shijian Xu
Article

DOI: 10.1079/IVP2006748

Cite this article as:
Wang, J., An, L., Wang, R. et al. In Vitro Cell.Dev.Biol.-Plant (2006) 42: 148. doi:10.1079/IVP2006748

Summary

A method was developed for in vitro regeneration of plants via somatic embryogenesis in Chorispora bungeana, an alpine plant with freeze-tolerance, using cell suspensions initiated from leaf-derived callus. Primary calli were induced from leaves of C. bungeana grown on Murashige and Skoog (MS) media supplemented with 4.0 mg l−1 gibberellic acid (GA3), 0.2 mgl−1 α-naphthaleneacetic acid (NAA) and 0.2 mgl−1 2,4-dichlorophenoxyacetic acid (2,4-D). Suspension culture was initiated by incubating the callus particulates in liquid MS medium supplemented with 1.0 mgl−1 kinetin (KT) and 0.2 mgl−1 NAA. Individual early cotyledonary-stage somatic embryos isolated from cell suspension developed into whole plants on medium containing high levels of sucrose (60 and 90 gl−1), whereas lower sucrose concentrations (0 and 30 gl−1) were inhibitory to main root development. On the MS medium with 90 gl−1 sucrose, one regenerated plant exhibited hetero-morphologic leaves, while other plants grown on different media showed a transformation from stem to root.

Key words

chromosome number somatic embryo sucrose concentration suspension cell culture tissue culture 

Copyright information

© Society for In Vitro Biology 2006

Authors and Affiliations

  • Jianhui Wang
    • 1
  • Lizhe An
    • 1
    • 2
  • Ruoyu Wang
    • 1
  • Daqun Yang
    • 2
  • Jing Si
    • 2
  • Xuanying Fu
    • 2
  • Jianfeng Chang
    • 2
  • Shijian Xu
    • 2
  1. 1.Cold and Arid Regions Environment and Engineering Research InstituteChinese Academy of SciencesLanzhouP.R. China
  2. 2.School of Life ScienceLanzhou UniversityLanzhouP.R. China

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