Stable genetic transformation of embryogenic cultures of Abies nordmanniana (nordmann fir) and regeneration of transgenic plants
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Stable genetic transformation of embryogenic cultures of Abies nordmanniana (Nordmann fir or Caucasian fir) was achieved using the Biolistic® transformation technology, followed by regeneration of transgenic plants. Selection of the transgenic tissue was based on the antibiotic resistance induced by the neomycin phosphotransferase II gene (npt II), in combination with the antibiotic geneticin. Six transclones were recovered from a total of 215 bombardments. Expression of the β-glucuronidase gene (uidA) was confirmed by histochemical analysis, and expression of npt II was verified by quantification of NPTII protein by enzyme linked immunosorbent assay (ELISA). Both genes were still expressed in the embryogenic tissue after 5 yr of in vitro culture and in mature somatic embryos and plants produced from these cultures. The integration of npt II was confirmed by Southern hybridization in embryogenic tissue after 5 yr of culture. After 5 yr of growth, uidA was still expressed in needles from the transformed trees.
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- Stable genetic transformation of embryogenic cultures of Abies nordmanniana (nordmann fir) and regeneration of transgenic plants
In Vitro Cellular & Developmental Biology - Plant
Volume 41, Issue 6 , pp 725-730
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Nordmann fir
- Abies nordmanniana
- Biolistic® transformation
- genetic engineering
- gene expression
- Industry Sectors
- Author Affiliations
- 1. New Zealand Forest Research Institute Ltd, Sala Street, Private Bag 3020, Rotorua, New Zealand
- 2. Tissue Culture Laboratory, Botanic Garden and Museum, Natural History Museum of Denmark, University of Copenhagen, DK-1353, Copenhagen K, Denmark