Stable genetic transformation of embryogenic cultures of Abies nordmanniana (nordmann fir) and regeneration of transgenic plants
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Stable genetic transformation of embryogenic cultures of Abies nordmanniana (Nordmann fir or Caucasian fir) was achieved using the Biolistic® transformation technology, followed by regeneration of transgenic plants. Selection of the transgenic tissue was based on the antibiotic resistance induced by the neomycin phosphotransferase II gene (npt II), in combination with the antibiotic geneticin. Six transclones were recovered from a total of 215 bombardments. Expression of the β-glucuronidase gene (uidA) was confirmed by histochemical analysis, and expression of npt II was verified by quantification of NPTII protein by enzyme linked immunosorbent assay (ELISA). Both genes were still expressed in the embryogenic tissue after 5 yr of in vitro culture and in mature somatic embryos and plants produced from these cultures. The integration of npt II was confirmed by Southern hybridization in embryogenic tissue after 5 yr of culture. After 5 yr of growth, uidA was still expressed in needles from the transformed trees.
- Bishop-Hurley, S. L.; Zabkievicz, R. J.; Grace, L. J.; Gardner, R. C.; Walter, C. Conifer genetic engineering: transgenic Pinus radiata (D Don) and Picea abies (Karst) plants are resistant to the herbicide Buster. Plant Cell Rep. 20:235–243; 2001. CrossRef
- Charest, P. J.; Devantier, Y.; Lachance, D. Stable transformation of Picea mariana (Black spruce) via microprojectile bombardment. In Vitro Cell. Dev. Biol. Plant 32:91–99; 1996. CrossRef
- Charity, J. A.; Holland, L.; Grace, L. J.; Walter, C. Consistent and stable expression of the npt II, uidA and bar genes in transgenic Pinus radiata after Agrobacterium tumefaciens-mediated transformation using nurse cultures. Plant Cell Rep. 23:606–616; 2005. CrossRef
- Doyle, J.; Doyle, J. Isolation of Plant DNA from fresh tissue, Focus 12:13–15; 1987.
- Ellis, D. D.; McCabe, D. E.; McInnis, S.; Ramachandran, R.; Russell, D. R.; Wallace, K. M.; Martinell, B. J.; Roberts, D. R.; Raffa, K. F.; McCown, B. H. Stable transformation of Picea glauca by particle acceleration. Bio/Technology 11:84–89; 1993. CrossRef
- Find, J.I. Culturing conifer embryonic cell mass in culture medium containing an anti-auxin improves maturation of conifer somatic embryos and plant propagation of coniferous tree, Patent no. WO200120972-A; 2001.
- Find, J. I.; Grace, L.; Krogstrup, P. Effects of anti-auxins on maturation of embryogenic tissue cultures of Nordmann fir (Abies nordmanniana). Physiol. Plant. 116:231–237; 2002. CrossRef
- Huang, Y.; Diner, A. M.; Karnosky, D. F. Agrobacterium rhizogenes-mediated genetic transformation and regeneration of a conifer: Larix decidua. In Vitro Cell Dev. Biol. Plant 27:201–207; 1991.
- Kay, R.; Chan, A.; Daly, M.; McPherson, J. Duplication of CaMV 35S promoter sequences creates a strong enhancer for plant genes. Science 236:1299–1302; 1987. CrossRef
- Klimaszewska, K.; Lachance, D.; Bernier-Cardou, M.; Rutlege, R. G. Transgene integration patterns and expression levels in transgenic lines of Picea mariana, P. glauca and P. abies. Plant Cell Rep. 21:1080–1087; 2003. CrossRef
- Klimaszewska, K.; Lachance, D.; Pelletier, G.; Lelu, A. M.; Seguin, A. Regeneration of transgenic Picea glauca, P. mariana and P. abies after cocultivation of embryogenic tissue with Agrobacterium tumefaciens. In Vitro Cell. Dev. Biol. Plant 37:748–755; 2001. CrossRef
- Kozak, M. The scanning model for translation: An update. J. Cell Biol. 109:229–241; 1989. CrossRef
- Levée, V.; Garin, E.; Klimaszewska, K.; Séguin, A. Stable genetic transformation of white pine (Pinus strobus L.) after cocultivation of embryogenic tissues with Agrobacterium tumefaciens. Mol. Breed. 5:429–440; 1999. CrossRef
- Levée, V.; Lelu, M.-A.; Jouanin, L.; Cornu, D.; Pilate, C. Agrobacterium tumefaciens-mediated transformation of hybrid larch (Larix kaempferi x L. decidua) and transgenic plant regeneration. Plant Cell Rep. 16:680–685; 1997. CrossRef
- Pena, L.; Séguin, A. Recent advances in the genetic transformation of trees. Trends Biotechnol. 19:500–506; 2001. CrossRef
- Tang, W.; Newton, R. J. Genetic transformation of conifers and its application in forest biotechnology. Plant Cell Rep. 22:1–15; 2003. CrossRef
- Wagner, A.; Moody, J.; Grace, L. J.; Walter, C. Transformation of Pinus radiata based on selection with hygromycin B. NZ J. For. Sci. 27(3):280–288; 1997.
- Walter, C.; Fenning, T. Deployment of genetically-engineered trees in plantation forestry—an issue of concern? The science and politics of genetically modified tree plantations. In: Walter, C.; Carson, M. J., eds. Plantation forest biotechnology for the 21st century, Kerala, India: Research Signpost; 2004;423–424.
- Walter, C.; Grace, L. J.; Donaldson, S. S.; Moody, J.; Gemmell, J. E.; van der Maas, S.; Kvaalen, H.; Lönneborg, A. An efficient Biolistic® transformation protocol for Picea abies (L.) Karst embryogeneic tissue and regeneration of transgenic plants. Can. J. For. Res. 29:1539–1546; 1999. CrossRef
- Walter, C.; Grace, L. J.; Wagner, A.; Walden, A. R.; White, D. W. R.; Donaldson, S. S.; Hinton, H. H.; Gardner, R. C.; Smith, D. R. Stable transformation and regeneration of transgenic plants of Pinus radiata D. Don. Plant Cell Rep. 17:460–468; 1998. CrossRef
- Walter, C.; Smith, D. R.; Connett, M. B.; Grace, L. J.; White, D. W. R. A biolistic approach for the transfer and expression of a uidA reporter gene in embryogenic cultures of Pinus radiata. Plant Cell Rep. 14:69–74; 1994. CrossRef
- Wenck, A. R.; Quinn, M.; Whetten, R. W.; Pullman, G.; Sederoff, R. High efficiency Agrobacterium-mediated transformation of Norway spruce (Picea abies) and loblolly pine (Pinus taeda). Plant Mol. Biol. 39:407–416; 1999. CrossRef
- Stable genetic transformation of embryogenic cultures of Abies nordmanniana (nordmann fir) and regeneration of transgenic plants
In Vitro Cellular & Developmental Biology - Plant
Volume 41, Issue 6 , pp 725-730
- Cover Date
- Print ISSN
- Online ISSN
- Additional Links
- Nordmann fir
- Abies nordmanniana
- Biolistic® transformation
- genetic engineering
- gene expression
- Industry Sectors
- Author Affiliations
- 1. New Zealand Forest Research Institute Ltd, Sala Street, Private Bag 3020, Rotorua, New Zealand
- 2. Tissue Culture Laboratory, Botanic Garden and Museum, Natural History Museum of Denmark, University of Copenhagen, DK-1353, Copenhagen K, Denmark