In Vitro Cellular & Developmental Biology - Plant

, Volume 41, Issue 6, pp 757–761

Simple one-medium formulation regeneration of fingerroot [Boesenbergia rotunda (L.) mansf. Kulturpfl.] via somatic embryogenesis

  • S. K. Tan
  • R. Pippen
  • R. Yusof
  • H. Ibrahim
  • N. Rahman
  • N. Khalid
Article

DOI: 10.1079/IVP2005695

Cite this article as:
Tan, S.K., Pippen, R., Yusof, R. et al. In Vitro Cell.Dev.Biol.-Plant (2005) 41: 757. doi:10.1079/IVP2005695

Summary

Most published protocols necessitate different media formulations for multistep somatic embryogenesis. This study aims to establish a simple but effective formulation for the regeneration of plantlets of the pharmaceutically active Boesenbergia rotunda (L.) Mansf. Kulturpfl, formerly Boesenbergia/Kaempferia pandurata (Schult), to ensure a superior and consistent supply of materials for commercialization purposes. In this study, a single-medium formulation of Murashige and Skoog (MS) supplemented with 13.54μM 2,4-dichlorophenoxyacetic acid (2,4-D) was found to be the only medium out of eight formulations to promote the complete somatic embryogenesis process for the culture of B. rotunda (L.). Callus cultures were initiated from a total of 280 explants of rhizome meristem. The percentage of cultures forming embryogenic callus was 23.3 ±4.3% on this MS medium augmented by 13.54μM 2,4-D. The best plantlet regeneration rate was attained from the first subcultured callus with a mean of 6.6±0.1 plantlets per 1 cm diameter aggregate of callus. Somatic embryogenesis characteristic of monocots was evident from histological studies. The regenerated plantlets have been successfully established in soil.

Key words

auxins micropropagation ginger Zingiberaceae 

Copyright information

© Society for In Vitro Biology 2005

Authors and Affiliations

  • S. K. Tan
    • 1
  • R. Pippen
    • 2
  • R. Yusof
    • 3
  • H. Ibrahim
    • 4
  • N. Rahman
    • 5
  • N. Khalid
    • 4
  1. 1.Sunway University CollegeSelangorMalaysia
  2. 2.Western Michigan UniversityKalamazoo
  3. 3.Biochemistry Department, Medical FacultyUniversity MalayaKuala LumpurMalaysia
  4. 4.Institute of Biological Sciences, Science FacultyUniversity MalayaKuala LumpurMalaysia
  5. 5.Chemistry Department, Science FacultyUniversity MalayaKuala LumpurMalaysia

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