In Vitro Cellular & Developmental Biology - Plant

, Volume 41, Issue 1, pp 58-62

First online:

Effects of the growth retardant paclobutrazol on large-scale micropropagation of daylily (Hemerocallis spp.)

  • Jianxin ChenAffiliated withDepartment of Biology, Brock University Email author 
  • , Dawn E. HallAffiliated withDepartment of Biology, Brock University
  • , Vincenzo De LucaAffiliated withDepartment of Biology, Brock University

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Meristematic clusters were induced from daylily scape explants (pedicel-scape junction) in the presence of the growth retardant Paclobutrazol on semisolid agar medium. Liquid shake culture was used to proliferate meristematic clusters. Highly efficient regeneration of adventitious shoots occurred on clusters after subculture on a 0.8% agar strength semisolid medium with the addition of activated charcoal. Paclobutrazol and sucrose levels in the media were found to significantly affect starch accumulation, growth value, and dry weight percentage of liquid-cultured meristematic clusters. The use of liquid shake cultures for mass proliferation of meristematic clusters followed by regeneration of adventitious shoots on semisolid agar culture could be an efficient system for large-scale micropropagation of daylily.

Key words

daylily Hemerocallis growth retardant liquid culture micropropagation