In Vitro Cellular & Developmental Biology - Plant

, Volume 41, Issue 1, pp 58–62

Effects of the growth retardant paclobutrazol on large-scale micropropagation of daylily (Hemerocallis spp.)

Article

DOI: 10.1079/IVP2004595

Cite this article as:
Chen, J., Hall, D.E. & De Luca, V. In Vitro Cell.Dev.Biol.-Plant (2005) 41: 58. doi:10.1079/IVP2004595

Summary

Meristematic clusters were induced from daylily scape explants (pedicel-scape junction) in the presence of the growth retardant Paclobutrazol on semisolid agar medium. Liquid shake culture was used to proliferate meristematic clusters. Highly efficient regeneration of adventitious shoots occurred on clusters after subculture on a 0.8% agar strength semisolid medium with the addition of activated charcoal. Paclobutrazol and sucrose levels in the media were found to significantly affect starch accumulation, growth value, and dry weight percentage of liquid-cultured meristematic clusters. The use of liquid shake cultures for mass proliferation of meristematic clusters followed by regeneration of adventitious shoots on semisolid agar culture could be an efficient system for large-scale micropropagation of daylily.

Key words

daylilyHemerocallisgrowth retardantliquid culturemicropropagation

Copyright information

© Society for In Vitro Biology 2005

Authors and Affiliations

  • Jianxin Chen
    • 1
  • Dawn E. Hall
    • 1
  • Vincenzo De Luca
    • 1
  1. 1.Department of BiologyBrock UniversitySt. CatharinesCanada