In Vitro Cellular & Developmental Biology - Plant

, Volume 40, Issue 5, pp 491–494

Effects of salinity on growth and compatible solutes of callus induced from Populus euphratica

  • F. Zhang
  • Y. L. Yang
  • W. L. He
  • X. Zhao
  • L. X. Zhang
Article

DOI: 10.1079/IVP2004546

Cite this article as:
Zhang, F., Yang, Y.L., He, W.L. et al. In Vitro Cell.Dev.Biol.-Plant (2004) 40: 491. doi:10.1079/IVP2004546

Summary

The present study aimed to evaluate the response to salinity of Populus euphratica, which is more salt-resistant than other poplar cultivars, at the cellular level. To this purpose, callus was induced from shoot segments of P. euphratica on Murashige and Skoog (MS) medium supplemented with 0.5 mg l−1 (2.2 μM) 6-benzyladenine (BA) and 0.5 mg l−1 (2.7 μM 1-naphthaleneacetic acid (NAA). Callus was transferred to MS medium supplemented with 0.25 mg l−1 (1.1 μM) BA and 0.5 mg l−1 NAA. The relative growth rate of callus reached a maximum in the presence of 50 mmol l−1 NaCl and growth was inhibited with increasing NaCl concentrations. Examination of the changes of osmotic substances under salt stress showed that accumulation of proline, glycine betaine, and total soluble sugars increased with increasing salt concentrations. The results indicate that the response of the callus of P. euphratica to salt stress is similar to that of the whole plant.

Key words

callusglycine betaineNaClprolinePopulus euphraticasalt stresssoluble sugar

Copyright information

© Society for In Vitro Biology 2004

Authors and Affiliations

  • F. Zhang
    • 1
    • 2
  • Y. L. Yang
    • 1
  • W. L. He
    • 1
  • X. Zhao
    • 1
  • L. X. Zhang
    • 1
    • 3
  1. 1.Key Lab of Desert and Desertification, Cold and Arid Regions Environmental and Engineering Research InstituteChinese Academy of SciencesLanzhouP.R. China
  2. 2.Faculty of AgricultureGansu Agricultural UniversityLanzhouP.R. China
  3. 3.School of Life Science, State Key Lab of Arid AgroecologyLanzhou UniversityLanzhouP.R. China