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Calluses of Cynara cardunculus Var. Cardunculus cardoon (Asteraceae): determination of cynarine and chlorogenic acid by automated high-performance capillary electrophoresis

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Summary

Cynara cardunculus var. cardunculus L., also known as cardoon, is a perennial weed naturalized in the Pampas region of Argentina. A quantification of cynarine and chlorogenic acid of callus and leaves from cardoon was performed by means of micellar electrokinetic capillary chromatography, showing that the content of cynarine is higher in calluses than in vivo leaves. The scavenging effect of the callus extract, determined by the thiobarbituric acid method, demonstrated its significant antioxidant capacity. The obtained results revealed that in vitro tissue culture is an excellent tool for producing cynarine for therapeutical purposes.

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References

  • Adzet, T.; Camarasa, J.; Laguna, J. C. Hepatoprotective activity of polyphenolic compounds from Cynara scolymus against CC14 toxicity in isolated rat hepatocytes. J. Nat. Prod. 50(4):612–617; 1987.

    Article  PubMed  CAS  Google Scholar 

  • Ariza Espinar, L.; Delucchi, G. Flora fanerogamica argentina—Asteraceae, Parte II. Tribu Cardueae. Conseio Nacional de Investigaciones Científicas y Tecnológicas (CONICET). Argentina Pro Flora 60:280; 1998.

    Google Scholar 

  • Ben-hod, G.; Basnizki, Y.; Zohary, D.; Mayer, A. M. Cynarine and chlorogenic acid content in germinating seeds of globe artichoke Cynara scolymus L. J. Genet. Breed. 46:63–68; 1992.

    CAS  Google Scholar 

  • Berregui, I.; Santos, J. I.; Del Campo, G.; Miranda, J. I.; Aizpurua, J. M. Quantitation determination of chlorogenic acid in cider apple juices by 1H NMR spectrometry. Anal. Chim. Acta 486:269–274; 2003.

    Article  CAS  Google Scholar 

  • Bombardelli, E.; Gabetta, B.; Martinelli, E. M., Gas-liquid chromatography and mass spectrometric investigations on Cynara scolymus L. extracts. Fitoterapia 4:143–151; 1977.

    Google Scholar 

  • Cordeiro, M.; Xue, Z. T.; Pais, M. S.; Brodelius, P. E. Proteases from cell suspension cultures of Cynara cardunculus. Phytochemistry 33(6):1323–1326; 1993.

    Article  CAS  Google Scholar 

  • De Fossard, R. A. Tissue culture for plant propagators. Armidale, Australia: Department of Botany, University of New England; 1976.

    Google Scholar 

  • Debenedetti, S. L.; Palacios, P. S.; Wilson, E. G.; Coussio, J. D. HPLC analysis of caffeoylquinic acids content in Argentine medicinal plants. Acta Hort. (ISHS) 333: 191–200; 1993.

    Google Scholar 

  • Faro, C. J.; Moir, A. J. G.; Pires, E. V. Specificity and kinetics of the milk clotting enzyme from cardoon (Cynara cardunculus L.) toward bovine K-casein. J. Agric. Food Chem. 41 (10):1537–1540; 1993.

    Article  Google Scholar 

  • Figueiredo, A. C.; Fevereiro, P.; Cabral, N. S.; Novais, J. N.; Pais, S. S. Callus and suspension cultures for biomass production of Cynara cardunculus L. (Compositae). Biotechnol. Lett. 9(3):213–218; 1987.

    Article  CAS  Google Scholar 

  • Gebhardt, R. Antioxidative and protective properties of extracts from leaves of the artichoke (Cynara scolymus L.) against hydroperoxide-induced oxidative stress in cultured rat hepatocytes. Toxicol. Appl. Pharmacol. 144(2):274–286; 1997.

    Article  Google Scholar 

  • Gebhardt, R. Inhibition of cholesterol biosynthesis in primary cultured rat hepatocytes by artichoke (Cynara scolymus L.) extracts. Pharm. Exp. Therap. 286(3):1122–1128; 1998.

    CAS  Google Scholar 

  • Grançal, D.; Nagy, M.; Suchy, V.; Novomesky, P. Cynarine from the fresh flower buds of Cynara cardunculus L. Fitoterapia 65(3):282; 1994.

    Google Scholar 

  • Inatani, R.; Nagy, M.; Suchy, V.; Novomesky, P. Cynarine from the fresh flower buds of Cynara cardunculus L. Fitoterapia 65(3):282; 1994.

    Google Scholar 

  • Inatani, R.; Nakatani, N.; Fuwa, H. Antioxidative effect of the constituents of Rosemary Rosmarinus officinalis L. and their derivation. Agric. Biol. Chem. 47(3):521–528; 1983.

    CAS  Google Scholar 

  • Kahl, R.; Kappus, H. Toxicology of the synthetic antioxidants BHA and BHT in comparison with the natural antioxidant vitamin E. Z. Lebensm. Unters. For. 196:329–338; 1993.

    Article  CAS  Google Scholar 

  • King, P. J.; Ma, G.; Miaow, W.; Jia, Q.; McDougall, B. R.; Reinecke, M. G.; Cornell, C.; Kuan, J.; Kim, T. R.; Robinson, W. E. Jr. Structure-activity relationships: analogues of the dicaffeoylquinic and dicaffeoyltartaric acids as potent inhibitors of human immunodeficiency virus type 1 integrase replication. J. Med. Chem. 42(3):497–509; 1999.

    Article  PubMed  CAS  Google Scholar 

  • Larson, R. A. The antioxidants of higher plants. Phytochemistry 27(4):969–978; 1988.

    Article  CAS  Google Scholar 

  • Llorach, R.; Espín, J. C.; Tomás-Barberán, F. A.; Ferreres, F. Artichoke (Cynara scolymus L.) bioproducts as a potential source of health-promoting antioxidant phenolics. J. Agr. Food Chem. 50:3458–3464; 2002.

    Article  CAS  Google Scholar 

  • Lorenzo, E.; Apóstolo, N. M.; Fernández, G. Cynara cardunculus L. cardo de Castilla: su utilización en la industria quesera y farmacéutica. Anales SAIPA 15:135–142; 1997.

    Google Scholar 

  • Martino, V. S.; Ferraro, C. E.; Debenedetti, S. L.; Coussio, J. D. Determinación espectrofotométrica del contenido de ácidos cafeoilquinicos en especies argentinas de Compuestas usadas en la medicina popular. Acta Farm. Boanerense 8(1):3–9; 1989.

    CAS  Google Scholar 

  • Mauri, P.; Pietta, P. Electrospray characterization of selected medicinal plants. Pharm. Biom. Anal. 23(1):61–68; 2000.

    Article  CAS  Google Scholar 

  • Morishita, H.; Iwahashi, H.; Osaka, N.; Kido, R. Chromatographic separation and identification of naturally occuring chlorogenic acids by 1H nuclear magnetic resonance spectroscopy and mass spectrometry. J. Chromatogr. 315:253–260; 1984.

    Article  PubMed  CAS  Google Scholar 

  • Mulinacci, N.; Prucher, D.; Peruzzi, M.; Romani, A.; Pinelli, P.; Giaccherini, C.; Vincieri, F. F. Commercial and laboratory extracts from artichoke leaves: estimation of caffeoyl esters and flavonoidic compounds content. J. Pharm. Biom. Anal. 34:349–357; 2004.

    Article  CAS  Google Scholar 

  • Murashige, T.; Skoog, F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15:473–492; 1962.

    Article  CAS  Google Scholar 

  • Panizzi, L.; Scarpatti, M. L. Isolamento e costituzione del principio attivo del carciofo. Gazzeta Chímica Italiana 84:792–805; 1954.

    CAS  Google Scholar 

  • Pauli, G. F.; Poetsch, F.; Nahrstedt, A. Structure assignment of natural quinic acid derivatives using proton nuclear magnetic resonance techniques. Phytochem. Anal. 9:177–185; 1998.

    Article  CAS  Google Scholar 

  • Pomilio, A. B.; Trajtemberg, S.; Vitale, A. A. High-Performance Capillary Electrophoresis analysis of mate infusions prepared from stems and leaves of Ilex paraguariensis using automated micellar electrokinetic capillary chromatography. Phytochem. Anal. 13:235–241; 2002.

    Article  PubMed  CAS  Google Scholar 

  • Sevciková, P.; Glatz, Z.; Slanina, J. Analysis of artichoke (Cynana cardunculus L.) extract by means of micellar electrokinetic capillary chromatography. Electrophoresis 23:249–252; 2002.

    Article  PubMed  Google Scholar 

  • Valentao, P.; Fernandes, E.; Carvalho, F.; Andrade, P. B.; Seabra, R. M.; Bastos, L. Antioxidative properties of cardoon (C. cardunculus L.) infusion against superoxide radical, hydroxyl radical, and hypochlorous acid. J. Agric. Food Chem. 50:4989–4993; 2002.

    Article  PubMed  CAS  Google Scholar 

  • Wang, M.; Simon, J. E.; Aviles, I. F.; He, K.; Zheng, Q.; Tadmor, Y. Analysis of antioxidative phenolic compounds in artichoke (C. scolymus L.). J. Agric. Food Chem. 51:601–608; 2003.

    Article  PubMed  CAS  Google Scholar 

  • Wiklund, A. The genus Cynara L. (Asteraceae—Cardueae). Bot. J. Lin. Soc. 109:75–123; 1992.

    Google Scholar 

  • Zhu, X.; Zhang, H.; Lo, R. Phenolic compounds from the leaf extract of artichoke (Cynara scolymus L.) and their antimicrobial activities. J. Agric. Food Chem. 52:7272–7278; 2004.

    Article  PubMed  CAS  Google Scholar 

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Correspondence to Nancy M. Apóstolo.

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Trajtemberg, S.P., Apóstolo, N.M. & Fernández, G. Calluses of Cynara cardunculus Var. Cardunculus cardoon (Asteraceae): determination of cynarine and chlorogenic acid by automated high-performance capillary electrophoresis. In Vitro Cell.Dev.Biol.-Plant 42, 534–537 (2006). https://doi.org/10.1079/IVP2006803

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