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Preliminary studies on Botryosphaeria species from Southern Hemisphere conifers in Australasia and South Africa

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Abstract

Wollemia nobilis is an ancient coniferous tree species that was recently discovered in eastern Australia. This tree is highly threatened due to its limited distribution. No genetic variation has been detected within the wild populations of ∼ 100 adult plants. A recent study has revealed that a species of Botryosphaeria is highly pathogenic to W. nobilis. The aim of the present study was to identify this fungus, as well as Botryosphaeria isolates of unknown identity from other Southern Hemisphere coniferous hosts, Araucaria from New Zealand and Widdringtonia from South Africa. To facilitate their identification, sequence data for the ITS rDNA, as well as the p-tubulin and translation elongation factor 1-α genes were combined to determine the phylogenetic relationship of these isolates with those of known Botryosphaeria spp. Isolates from W. nobilis included two Botryosphaeria spp. The first is closely related to B. ribis, but also shares some unique sequence polymorphisms with B. parva. One isolate grouped with B. australis, but also varied slightly from this taxon in the gene regions analysed. Additional isolates will be needed to determine whether these sequence variations represent speciation events or merely variation within populations of B. ribis and B. australis. In addition to this, B. parva was identified from Araucaria in New Zealand, and B. australis was found on Widdringtonia trees in South Africa. All three reports of these fungi are new records for their various hosts and could represent important pathogens of these trees.

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References

  • Bullock S, Summerell BA, Gunn LV (2000) Pathogens of the Wollemi Pine, Wollemia nobilis. Australasian Plant Pathology29, 211–214.

    Article  Google Scholar 

  • Burgess T, Wingfield MJ (2002) Quarantine is important in restricting the spread of exotic seed-borne tree pathogens in the Southern Hemisphere. International Forestry Review4, 56–65.

    Google Scholar 

  • Davis JI, Nixon KC (1992) Populations, genetic variation, and the delimitation of phylogenetic species. Systematic Biology41, 421–435.

    Article  Google Scholar 

  • Davison EM, Tay CS (1983) Twig, branch and upper trunk cankers of Eucalyptus marginata. Plant Disease67, 1285–1287.

    Article  Google Scholar 

  • Denman S, Crous PW, Groenewald JZ, Slippers B, Wingfield BD, Wingfield MJ (2003) Circumscription of Botryosphaeria species associated with Proteaceae on morphology and DNA sequence data. Mycologia95, 294–307.

    Article  CAS  Google Scholar 

  • Denman S, Crous PW, Taylor IE, Kang JC, Pascoe I, Wingfield MJ (2000) An overview of the taxonomic history of Botryosphaeria and a re-evaluation of its anamorphs based on morphology and ITS rDNA phylogeny. Studies in Mycology45, 129–140.

    Google Scholar 

  • Eriksson T (1998) Autodecay ver. 4.0 (program distributed by the author). (Department of Botany, Stockholm University: Stockholm)

    Google Scholar 

  • Farris JS, Kallersjo M, Kluge AG, Bult C (1994) Testing significance of incongruence. Cladistics10, 315–319. doi: 10.1006/ clad. 1994.1021

    Article  Google Scholar 

  • Felsenstein J (1985) Confidence intervals on phylogenetics: an approach using bootstrap. Evolution; International Journal of Organic Evolution39, 783–791.

    Article  Google Scholar 

  • Gilmore S, Hill KD (1997) Relationships of the Wol lemi pine ( Wollemia nobilis) and a molecular phylogeny of the Araucariaceae. Telopea7, 275–291.

    Article  Google Scholar 

  • Hill KD (1997) The Wollemi Pine — a recently discovered Australian genus of Araucariaceae. Botany84, 202–203 [abstract].

    Google Scholar 

  • Hillis DM, Huelsenbeck JP (1992) Signal, noise, and reliability in molecular phylogenetic analyses. The Journal of Heredity83, 189–195.

    Article  CAS  Google Scholar 

  • Hogbin PM, Peakall R, Sydes MA (2000) Achieving practical outcomes from genetic studies of rare Australian plants. Australian Journal of Botany48, 375–382. doi: 10.1071/BT98080

    Article  Google Scholar 

  • Huelsenbeck JP, Bull JJ, Cunningham CW (1996) Combining data in phylogenetic analysis. Trends in Ecology & Evolution11, 152–158. doi: 10.1016/0169-5347(96)10006-9

    Article  CAS  Google Scholar 

  • Jacobs KA, Rehner SA (1998) Comparison of cultural and morphological characters and ITS sequences in anamorphs of Botryosphaeria and related taxa. Mycologia90, 601–610.

    Article  CAS  Google Scholar 

  • Jones WG, Hill KD, Allen JM (1995) Wollemia nobilis, a new living Australian genus and species in the Araucariaceae. Telopea6, 173–176.

    Article  Google Scholar 

  • Mustart P, Bond W (1995) The decline of the Clanwilliam cedar — natural or man made? Africa — Environment & Wildlife2, 80–81.

    Google Scholar 

  • Offord CA (1996) Conserving the Wollemi pine: an integrated approach. Danthonia5, 12–14.

    Google Scholar 

  • Offord CA, Porter CL, Meagher PF, Errington G (1999) Sexual reproduction and early plant growth of the Wollemi pine (Wollemia nobilis), a rare and threatened Australian conifer. Annals of Botany84, 1–9. doi: 10.1006/anbo. 1999.0882

    Article  Google Scholar 

  • Old KM, Gibbs R, Craig I, Myers BJ, Yaun ZQ (1990) Effect of drought and defoliation on the susceptibility of Eucalyptus to cankers caused by Endothia gyrosa and Botryosphaeria ribis. Australian Journal of Botany38, 571–581.

    Article  Google Scholar 

  • Pauw CA, Linder HP (1997) Tropical African cedars (Widdringtonia, Cuppressaceae): systematics, ecology and conservation status. Botanical Journal of the Linnean Society123, 297–319. doi: 0.1006/bojl. 1996.0091

    Article  Google Scholar 

  • Peakall R, Ebert D, Scott LJ, Meagher PF, Offord CA (2003) Comparative genetic study confirms exceptionally low genetic variation in the ancient and endangered relictual conifer, Wollemia nobilis (Araucariaceae). Molecular Ecology12, 2331–2343. doi: 10.1046/j.l365-294X.2003.01926.x

    Article  CAS  Google Scholar 

  • Pennycook SR, Samuels GJ (1985) Botryosphaeria and Fusicoccum species associated with ripe fruit rot of Actinidia deliciosa (Kiwifruit) in New Zealand. Mycotaxon24, 445–458.

    Google Scholar 

  • Phillips AJL, Fonseca F, Povoa V, Castilho R, Nolasco G (2002) A reassessment of the anamorphic fungus Fusicoccum luteum and description of its teleomorph Botryosphaeria lutea sp. nov. Sydowia54, 59–77.

    Google Scholar 

  • Raeder U, Broda P (1985) Rapid preparation of DNA from filamentous fungi. Letters in Applied Microbiology1, 17–20.

    Article  CAS  Google Scholar 

  • Shearer BL, Smith IW (2000) Diseases of eucalypts caused by Phytophthora and Pythium. In ‘Diseases and pathogens of Eucalyptus’. (Eds PJ Keane, GA Kile, FD Podger, BN Brown) (CSIRO Publishing: Melbourne, Australia)

    Google Scholar 

  • Shearer BL, Tippett JT, Bartle JR (1987) Botryosphaeria ribis infection associated with death of Eucalyptus radiata in species selection trials. Plant Disease71, 140–145.

    Article  Google Scholar 

  • Slippers B (2003) The taxonomy, phylogeny and ecology of Botryosphaeriaceous fungi occurring on various woody hosts. PhD Thesis, University of Pretoria, Pretoria, South Africa.

    Google Scholar 

  • Slippers B, Crous PW, Denman S, Coutinho TA, Wingfield BD, Wingfield MJ (2004a) Combined multiple gene genealogies and phenotypic characters differentiate several species previously identified as Botryosphaeria dothidea. Mycologia96, 83–101.

    Article  CAS  Google Scholar 

  • Slippers B, Fourie G, Crous PW, Coutinho TA, Wingfield BD, Wingfield MJ (2004b) Multiple gene sequences delimit Botryosphaeria australis sp. nov. from B. lutea. Mycologia96, 1028–1039.

    Article  Google Scholar 

  • Slippers B, Fourie G, Crous PW, Coutinho TA, Wingfield BD, Carnegie A, Wingfield MJ (2004c) Speciation and distribution of Botryosphaeria spp. on native and introduced Eucalyptus trees. Studies in Mycology50, 343–358.

    Google Scholar 

  • Slippers B, Johnston GI, Crous PW, Coutinho TA, Wingfield BD, Wingfield MJ (2005) Phylogenetic and morphological re-evaluation of the Botryosphaeria species causing diseases of Mangifera indica in Australia. Mycologia97, 102–113.

    Article  Google Scholar 

  • Smith H, Crous PW, Wingfield MJ, Coutinho TA, Wingfield BD (2001) Botryosphaeria eucalyptorum sp. nov, a new species in the B. dothidea -complex on Eucalyptus in South Africa. Mycologia93, 277–284.

    Article  Google Scholar 

  • Smith H, Wingfield MJ, Crous PW, Coutinho TA (1996) Sphaeropsis sapinea and Botryosphaeria dothidea endophytic in Pinus spp. and Eucalyptus spp. in South Africa. South African Journal of Botany62, 86–88.

    Article  Google Scholar 

  • Sutton BC, Dyko BJ (1989) Revision of Hendersonula. Mycological Research93, 466–488.

    Article  Google Scholar 

  • Swofford DL (1999) ‘PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4’. (Sinauer Associates: Sunderland, Massachusetts)

    Google Scholar 

  • Wingfield MJ, Von Broembsen SL, Manders PT (1988) A preliminary assessment of the threat of diseases and pests to Widdringtonia cedarbergensis. SA Forestry Journal147, 32–34.

    Google Scholar 

  • Young HE (1948) Hendersonula agathi n. sp., the cause of leaf cast of Kauri Pine in Queensland. Queensland Journal of Agricultural Science5, 9–12.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South Africa

    Bernard Slippers, Pedro W. Crous, Teresa A. Coutinho & Michael J. Wingfield

  2. Department of Genetics, Forestry and Agricultural Biotechnology Institute, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South Africa

    Brenda D. Wingfield

  3. Royal Botanic Gardens and Domain Trust, Mrs Macquaries Road, 2000, Sydney, NSW, Australia

    Brett A. Summerel

  4. Centraalbureau voor Schimmelcultures, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands

    Pedro W. Crous

Authors
  1. Bernard Slippers
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  2. Brett A. Summerel
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  3. Pedro W. Crous
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  4. Teresa A. Coutinho
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  5. Brenda D. Wingfield
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  6. Michael J. Wingfield
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Correspondence to Bernard Slippers.

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Slippers, B., Summerel, B.A., Crous, P.W. et al. Preliminary studies on Botryosphaeria species from Southern Hemisphere conifers in Australasia and South Africa. Australasian Plant Pathology 34, 213–220 (2005). https://doi.org/10.1071/AP05020

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