, Volume 32, Issue 2, pp 213-218

Detection of novel genotypes in progeny from a controlled cross between isolates of Uncinula necator belonging to distinct phenetic groups

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Sexual recombination and segregation in 18 ascospore progeny of Uncinula necator were analysed by mating type (Mat locus), RFLP and PCR markers. Progeny were analysed from a cross of parental isolates representing the genetically distinct phenetic Groups I (AHd2) and II (BNb2). A total of 27 markers was analysed, consisting of four single loci (Mat locus and three RFLP probes), 16 multi-loci (three multi-copy fingerprinting probes) and seven PCR loci. Novel hybrid genotypes were detected in 28% of the ascospore-derived progeny using the multi-copy RFLP probes, pUnl22-11 and pUnP27, and the PCR primers (CAC)5 and R1, confirming that recombination can occur between isolates representative of the two phenetic groups. This study provides evidence that out-crossing between isolates of U. necator generates novel genotypes and, as such, may have important implications for disease management practices, such as the durability of fungicides and host resistance.