Environmental Science and Pollution Research

, Volume 12, Issue 6, pp 361–368

Bacterial Community Structure Analyses to Assess Pollution of Water and Sediments in the Lake Shkodra/Skadar, Balkan Peninsula (8 pp)

  • Rok Kostanjsek
  • Aleš Lapanje
  • Damjana Drobne
  • Svetlana Nikcevic
  • Andrej Perović
  • Primož Zidar
  • Jasna Štrus
  • Henner Hollert
  • Gordan Karaman
Article

DOI: 10.1065/espr2005.07.271

Cite this article as:
Kostanjsek, R., Lapanje, A., Drobne, D. et al. Env Sci Poll Res Int (2005) 12: 361. doi:10.1065/espr2005.07.271

Abstract

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Goal, Scope, Background. Lake Skadar is the largest lake in Balkan Peninsula, located on the Montenegro-Albanian border. The unique features of the lake and wide range of endemic and rare or endangered plant and animal species resulted in the classification of the Skadar as a wetland site of international significance. In spite of its importance the Lake is influenced by inflowing waters from river Morača and other regional rivers contaminated by the industry, municipal and agricultural activities in the area. Therefore, the Lake has been subject of various physical, chemical, biological and toxicological examinations. However, community-level analyses are most relevant to assess the effect of stressors on aquatic ecosystems. In the present study bacterial community structure among differently polluted sites of the lake was compared by genetic fingerprinting technique.

Methods

Water and sediment samples were collected from five differently polluted sampling sites on the Lake Skadar in spring and autumn of the same year. The bacterial community structure in the samples was characterized and compared by temporal temperature gel electrophoresis (TTGE) analysis of polymerase chain reaction-amplified bacterial 16S rRNA genes.

Results and Discussion

The TTGE analysis resulted in many distinguishable and reproducible band patterns, allowing reliable comparison of bacterial communities among sampling sites. Results on the bacterial community structure revealed that three of the selected locations can be considered as sites that have not shown any pollution degradation determined by our method, due to similar structure of bacterial community in the sediment samples. On the other hand, significant shifts in bacterial community structure in the mouth of the river Morača and Plavnica were shown. Since the results coincide with some of the bioassays and chemical analysis performed previously, the changes in bacterial community structure are explained as an effect of antropogenic pollution on the lake ecosystem by waters of river Morača and stream Plavnica.

Conclusion

The TTGE has proven to be an efficient and reliable method to monitor bacterial dynamics and community shifts in aquatic environment, especially in the sediments. Within the variety of environmental quality assessments the use of TTGE analyses of bacterial community is strongly recommended, particularly as an initial investigation. However, in any conclusion on the state of the environment, the TTGE results should be combined to some other biological, chemical and hydrological data.

Recommendation and Outlook

Since prokaryotes are a crucial group of organisms in the biosphere, the ecosystem function studies are largely based on bacterial communities. Therefore, bacterial community structure analysis should be a part of an integrated weight of evidence approach in pollution assessment. In case of Triad approach, consisting of chemical analyses, bioassays, and community studies in the field, the TTGE bacterial community structure analyses should be placed in the later Triad leg. In comparison to other community studies, based on various biotic indices, the TTGE bacterial community analysis has proven to be very sensitive, reliable and less time consuming.

surface water Triad pollution sediments Lake Skadar bacterial community structure analysis temporal temperature gel electrophoresis (TTGE) 

Copyright information

© Ecomed 2005

Authors and Affiliations

  • Rok Kostanjsek
    • 1
  • Aleš Lapanje
    • 2
  • Damjana Drobne
    • 3
  • Svetlana Nikcevic
    • 4
  • Andrej Perović
    • 5
  • Primož Zidar
    • 6
  • Jasna Štrus
    • 7
  • Henner Hollert
    • 8
  • Gordan Karaman
    • 9
  1. 1.Dr. Rok Kostanjsek Department of Biology Biotechnical Faculty University of Ljubljana Vecna pot 111 1000 Ljubljana Slovenia rok.kostanjsek@bf.uni-lj.si
  2. 2.Aleš Lapanje University of Ljubljana Biotechnical Faculty Department of Biology Večna Pot 111 1000 Ljubljana SLOVENIA
  3. 3.Damjana Drobne University of Ljubljana Biotechnical Faculty Department of Biology Večna Pot 111 1000 Ljubljana SLOVENIA
  4. 4.Svetlana Nikcevic University of Montenegro Department of Biology Cetinski put bb 81000 Podgorica SERBIA AND MONTENEGRO
  5. 5.Andrej Perović University of Montenegro Department of Biology Cetinski put bb 81000 Podgorica SERBIA AND MONTENEGRO
  6. 6.Primož Zidar University of Ljubljana Biotechnical Faculty Department of Biology Večna Pot 111 1000 Ljubljana SLOVENIA
  7. 7.Jasna Štrus University of Ljubljana Biotechnical Faculty Department of Biology Večna Pot 111 1000 Ljubljana SLOVENIA
  8. 8.Dr. Henner Hollert Heidelberger Institut für Zoologie (HIZ) Universität Heidelberg Im Neuenheimer Feld 230 69120 Heidelberg Germany
  9. 9.Gordan Karaman University of Montenegro Department of Biology Cetinski put bb 81000 Podgorica SERBIA AND MONTENEGRO

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